Preparation, characterization and application of levan/montmorillonite biocomposite and levan/BSA nanoparticle

Carbohydr Polym. 2020 Apr 15:234:115921. doi: 10.1016/j.carbpol.2020.115921. Epub 2020 Jan 30.

Abstract

Here, two kinds of polysaccharide-based biocomposites were investigated. The enzymatically synthesized levan from Erwinia amylovora was applied as the matrix, while montmorillonite clay and bovine serum albumin (BSA) were used as additive in the biocomposite. To examine the properties of levan/MMT biocomposite, we choose different ratios between levan and MMT to implement the surface morphology observation, thermal property analysis, and rheological behavior determination. As a result, the levan/MMT biocomposite in a 2:1 blending ratio showed a significant improvement both in the thermal and rheological properties. Meanwhile, the 0.1 % levan/BSA nanoparticle showed the highest encapsulation capacity and surface charge as 53.13 ± 2.64 % and +3.92 ± 0.43 mV. Last but not least, the levan/BSA nanoparticle exhibited a slower and controlled release of the BSA from the system. All of these results indicated a potential application of levan-based biocomposite and nanoparticle.

Keywords: Application; BSA; Biocomposite; Levan; Montmorillonite; Nanoparticle.

MeSH terms

  • Animals
  • Bentonite / chemistry*
  • Biocompatible Materials / chemical synthesis
  • Biocompatible Materials / chemistry*
  • Cattle
  • Erwinia amylovora / enzymology
  • Fructans / biosynthesis
  • Fructans / chemistry*
  • Hexosyltransferases / metabolism
  • Nanoparticles / chemistry*
  • Particle Size
  • Recombinant Proteins / metabolism
  • Serum Albumin, Bovine / chemistry*
  • Surface Properties

Substances

  • Biocompatible Materials
  • Fructans
  • Recombinant Proteins
  • Bentonite
  • Serum Albumin, Bovine
  • levan
  • Hexosyltransferases
  • levansucrase