Synthesis of silver nanoparticles using gallic acid-conjugated chitosan derivatives

Alexey Lunkov; Balzhima Shagdarova; Mariya Konovalova; Yuliya Zhuikova; Natalia Drozd; Alla Il'ina; Valery Varlamov

doi:10.1016/j.carbpol.2020.115916

Synthesis of silver nanoparticles using gallic acid-conjugated chitosan derivatives

Carbohydr Polym. 2020 Apr 15:234:115916. doi: 10.1016/j.carbpol.2020.115916. Epub 2020 Jan 25.

Authors

Alexey Lunkov¹, Balzhima Shagdarova², Mariya Konovalova³, Yuliya Zhuikova², Natalia Drozd⁴, Alla Il'ina², Valery Varlamov²

Affiliations

¹ Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, 119071, Russian Federation. Electronic address: enzyme@biengi.ac.ru.
² Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, 119071, Russian Federation.
³ Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, 117997, Russian Federation.
⁴ National Research Center for Hematology, Moscow, 125167, Russian Federation.

PMID: 32070535
DOI: 10.1016/j.carbpol.2020.115916

Abstract

In presented study, various chitosan derivatives containing covalently bounded gallic acid were obtained: chitosan with gallic acid (CG), quaternized chitosan with gallic acid (QCG), and succinylated chitosan with gallic acid (SCG). Chitosan derivatives were used as stabilizing and reducing agents in the synthesis of silver nanoparticles (AgNPs). The dimensional characteristics of nanomaterials were determined by transmission electron (TEM), dynamic light scattering (DLS) and atomic force (AFM) microscopy, antibacterial activity (against E. coli, S. epidermidis), cytotoxicity (HaCaT, Colo 357 cell lines) and hemocompatibility. Among all samples, QCG-AgNPs showed low toxicity in the range of studied concentrations (3.125-100 μg/ml) high stability of nanoparticle for 4 months (according to UV.spectroscopy data) the highest antibacterial activity against S. epidermidis (3.91 μg/ml). The high antibacterial activity, stability, and simplicity of the process of producing AgNPs based on the QCG derivative reveals that a new method for producing modified AgNPs deserves future consideration.

Keywords: Antibacterial activity; Chitosan; Cytotoxicity; Gallic acid; Hemocompatibility; Silver nanoparticles.