Abstract
We introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling the cellular localization of GFP fusion proteins, the engineered nanobody allows interrogation of their roles in basic biological processes, an approach that should be applicable to numerous previously described GFP fusions. We also outline how the binding affinities of other nanobodies can be controlled by small molecules.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Crystallography, X-Ray
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DNA / chemistry
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Databases, Protein
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Escherichia coli
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Fluorescence Resonance Energy Transfer
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Gene Products, gag / chemistry
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Green Fluorescent Proteins / chemistry*
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HEK293 Cells
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HIV-1 / chemistry
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HeLa Cells
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Humans
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Immunoglobulin Fragments / chemistry*
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Kinetics
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Ligands
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Microscopy, Fluorescence
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Mitosis
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Nanoparticles / chemistry*
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Protein Domains
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Single-Domain Antibodies / chemistry*
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nef Gene Products, Human Immunodeficiency Virus / chemistry
Substances
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Gene Products, gag
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Immunoglobulin Fragments
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Ligands
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Single-Domain Antibodies
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nef Gene Products, Human Immunodeficiency Virus
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Green Fluorescent Proteins
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DNA