Identification of begomoviruses from different cryptic species of Bemisia tabaci in Bangladesh

Mst Fatema Khatun; Hwal-Su Hwang; Jae-Kyoung Shim; Eui-Joon Kil; Sukchan Lee; Kyeong-Yeoll Lee

doi:10.1016/j.micpath.2020.104069

Identification of begomoviruses from different cryptic species of Bemisia tabaci in Bangladesh

Microb Pathog. 2020 Feb 13:142:104069. doi: 10.1016/j.micpath.2020.104069. Online ahead of print.

Authors

Mst Fatema Khatun¹, Hwal-Su Hwang², Jae-Kyoung Shim², Eui-Joon Kil³, Sukchan Lee³, Kyeong-Yeoll Lee⁴

Affiliations

¹ Division of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, Republic of Korea; Department of Entomology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Dhaka, Bangladesh.
² Division of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, Republic of Korea; Institute of Plant Medicine, Kyungpook National University, Daegu, Republic of Korea.
³ Department of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of Korea.
⁴ Division of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, Republic of Korea; Institute of Plant Medicine, Kyungpook National University, Daegu, Republic of Korea; Institute of Agricultural Science and Technology, Kyungpook National University, Daegu, Republic of Korea. Electronic address: leeky@knu.ac.kr.

PMID: 32061918
DOI: 10.1016/j.micpath.2020.104069

Abstract

Bemisia tabaci is a global species complex consisting of at least 40 cryptic species. It is also a vector for at least 100 species of begomovirus, many of which cause severe crop damage. The relationship between begomoviruses and cryptic species of the B. tabaci species complex, however, remains unclear. Our previous study [13] was identified four cryptic species (Asia I, Asia II 1, Asia II 5, and Asia II 10) of B. tabaci from Bangladesh. Using those 110 whitefly samples, vector-based PCR analysis identified 8 different begomovirus species: BYVMV, BGYVV, OELCV, SLCCV, SLCV, TbCSV, ToLCBV, and ToLCNDV. The overall rate of virus infection was 26.4%, and BYVMV and ToLCNDV were the most frequently detected in the B. tabaci vector. Virus infection rates for Asia I, Asia II 1, Asia II 5, and Asia II 10 were 22.4% (15/67), 35% (7/20), 27.3% (6/22), and 100% (1/1), respectively. Each cryptic species infected multiple virus species, but SLCCV, TbCSV, and BGYVV were each only detected in, Asia I, Asia II 1, and Asia II 5, respectively. This study demonstrates the geographic distribution of various begomoviruses in Bangladesh and their relationships with cryptic species of B. tabaci.

Keywords: Begomovirus; Cryptic species; Genetic diversity; Vector; Whitefly.