Curcumin ameliorates Staphylococcus aureus-induced mastitis injury through attenuating TLR2-mediated NF-κB activation

Jin Xu; Zhifeng Jia; Aorigele Chen; Chunjie Wang

doi:10.1016/j.micpath.2020.104054

Curcumin ameliorates Staphylococcus aureus-induced mastitis injury through attenuating TLR2-mediated NF-κB activation

Microb Pathog. 2020 Feb 13:142:104054. doi: 10.1016/j.micpath.2020.104054. Online ahead of print.

Authors

Jin Xu¹, Zhifeng Jia², Aorigele Chen³, Chunjie Wang⁴

Affiliations

¹ College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, 010018, PR China; Henan Houyi Bio-Engineering, Inc., Zheng Zhou, 451161, PR China.
² College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, 010018, PR China.
³ College of Animal Science, Inner Mongolia Agricultural University, Hohhot, 010018, PR China.
⁴ College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, 010018, PR China. Electronic address: chunjiewang200@sohu.com.

PMID: 32061917
DOI: 10.1016/j.micpath.2020.104054

Abstract

Problem: It has long been known that Staphylococcus aureus (S. aureus, serotype CP8) is associated with clinical mastitis in cows, and recent scientific studies have shown that curcumin (CUR) is effective in anti-inflammatory. However, the mechanism of action of curcumin on S. aureus-induced cows has not been fully understood. Therefore, this study investigated whether curcumin could improve the inflammation response in mice mastitis and to clarify the possible mechanism.

Method: of study: A mouse mastitis model was established. The mice were administered curcumin (125 mg/kg), ciprofloxacin (130 mg/kg, CIP), and water (model group) for 5 days.

Results: CUR and CIP treatment prevented the S. aureus-induced mouse mastitis increase the levels of IL-2, IL-10, and IFN-γ and decrease levels of IL-6, IL-8, and TNF-α. Additionally, RT-PCR results showed that 20 μg/mL curcumin inhibited the mRNA expression of TNF-α, IL-6, IL-1β, TRAF6 and MEKK1 in murine mammary epithelial cells (MMECs). Likewise, Western blotting results showed that CUR inhibited the expression of TRAF6 and MEKK1.

Conclusion: These results indicated that CUR is superior to CIP in the prevention of mastitis, and the mechanism may be that the curative effect of CUR inhibits TLR-2 mediated NF-κB signaling pathway in mouse mastitis.

Keywords: Curcumin; Inflammation; Mastitis; NF-κB; Staphylococcus aureus; TLR-2.