Evaluation of the performance of Acuitas® Resistome Test and the Acuitas Lighthouse® software for the detection of β-lactamase-producing microorganisms

Evangelia Voulgari; Georgios Miliotis; Eirini Siatravani; Leonidas S Tzouvelekis; Eva Tzelepi; Vivi Miriagou

doi:10.1016/j.jgar.2020.01.017

Evaluation of the performance of Acuitas® Resistome Test and the Acuitas Lighthouse® software for the detection of β-lactamase-producing microorganisms

J Glob Antimicrob Resist. 2020 Sep:22:184-189. doi: 10.1016/j.jgar.2020.01.017. Epub 2020 Feb 13.

Authors

Evangelia Voulgari¹, Georgios Miliotis¹, Eirini Siatravani¹, Leonidas S Tzouvelekis², Eva Tzelepi¹, Vivi Miriagou³

Affiliations

¹ Laboratory of Bacteriology, Hellenic Pasteur Institute, Athens, Greece.
² Laboratory of Bacteriology, Hellenic Pasteur Institute, Athens, Greece; Department of Microbiology, Medical School, University of Athens, Athens, Greece.
³ Laboratory of Bacteriology, Hellenic Pasteur Institute, Athens, Greece. Electronic address: miriagou@pasteur.gr.

PMID: 32061878
DOI: 10.1016/j.jgar.2020.01.017

Abstract

Objectives: Given the international spread of multidrug-resistant Gram-negative bacteria the need for prompt and precise characterization of underlying resistance traits has evolved into the cornerstone of infection control strategies. Novel commercial molecular tests enable rapid simultaneous testing for multiple resistance genes. We aimed to evaluate the performance of OpGen's Acuitas® Resistome Test and the Acuitas Lighthouse® software.

Methods: The test is tailored towards detecting 46 β-lactamase genes (SHV and TEM variants associated with wild-type penicillin resistance, extended-spectrum β-lactamases [ESBLs], acquired AmpCs and carbapenemases) via a microfluidic polymerase chain reaction (PCR) array. In total 118 isolates part of the collection of the Bacteriology Laboratory of the Hellenic Pasteur Institute, specifically 96 enterobacterial isolates and 21 Acinetobacter baumannii, of divergent origins, with previously characterized β-lactamase content, were tested.

Results: In the enterobacterial group all 69 carbapenemase genes of the KPC, VIM, NDM and OXA-48 types were correctly identified (sensitivity, specificity, positive predictive value [PPV] and negative predictive value [NPV] of 100%). Non-ESBL SHV enzymes, ESBLs (CTX-M, GES, VEB types) and acquired AmpC enzymes were also correctly characterized. Of the 35 SHV-ESBLs harboured, correct identification was possible in 32/35 isolates, with overall sensitivity, specificity, PPV and NPV for the Klebsiella pneumoniae group of 89.29%, 100%, 100% and 91.18%, respectively. For the A. baumannii group the test exhibited an overall sensitivity for carbapenemase detection of 96.55% and 100% PPV.

Conclusions: The OpGen Acuitas Resistome Test is an efficient molecular tool that can identify resistance threats in health care institutions with high diagnostic accuracy and be integrated into targeted surveillance protocols.

Keywords: Acinetobacter baumannii; Acuitas Resistome Test; Enterobacteria; Molecular detection; β-lactamases.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acinetobacter baumannii* / genetics
Enterobacteriaceae / genetics
Klebsiella pneumoniae
Software
beta-Lactamases* / genetics

Substances

beta-Lactamases