Moesin facilitates metastasis of hepatocellular carcinoma cells by improving invadopodia formation and activating β-catenin/MMP9 axis

Shubing Lan; Xiaoyuan Zheng; Ping Hu; Xiaohua Xing; Kun Ke; Fei Wang; Niangmei Cheng; Qiuyu Zhuang; Xiaolong Liu; Jingfeng Liu; Bixing Zhao; Yingchao Wang

doi:10.1016/j.bbrc.2020.01.157

Moesin facilitates metastasis of hepatocellular carcinoma cells by improving invadopodia formation and activating β-catenin/MMP9 axis

Biochem Biophys Res Commun. 2020 Apr 16;524(4):861-868. doi: 10.1016/j.bbrc.2020.01.157. Epub 2020 Feb 10.

Authors

Shubing Lan¹, Xiaoyuan Zheng², Ping Hu², Xiaohua Xing³, Kun Ke⁴, Fei Wang⁴, Niangmei Cheng⁴, Qiuyu Zhuang³, Xiaolong Liu³, Jingfeng Liu³, Bixing Zhao⁵, Yingchao Wang⁶

Affiliations

¹ College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, PR China; The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, Fujian, 350025, PR China; Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, 350002, PR China.
² The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, Fujian, 350025, PR China; The Liver Center of Fujian Province, Fujian Medical University, Fuzhou, Fujian, 350025, PR China.
³ The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, Fujian, 350025, PR China; Mengchao Med-X Center, Fuzhou University, Fuzhou, Fujian, 350116, PR China; The Liver Center of Fujian Province, Fujian Medical University, Fuzhou, Fujian, 350025, PR China.
⁴ The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, Fujian, 350025, PR China; Mengchao Med-X Center, Fuzhou University, Fuzhou, Fujian, 350116, PR China.
⁵ The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, Fujian, 350025, PR China; Mengchao Med-X Center, Fuzhou University, Fuzhou, Fujian, 350116, PR China; The Liver Center of Fujian Province, Fujian Medical University, Fuzhou, Fujian, 350025, PR China. Electronic address: bixingzhao@gmail.com.
⁶ The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, Fujian, 350025, PR China; Mengchao Med-X Center, Fuzhou University, Fuzhou, Fujian, 350116, PR China; The Liver Center of Fujian Province, Fujian Medical University, Fuzhou, Fujian, 350025, PR China. Electronic address: Yingchaowang@live.com.

PMID: 32057364
DOI: 10.1016/j.bbrc.2020.01.157

Abstract

Moesin has been proved to be implicated in invasiveness and metastasis in many other cancers, but unclear in HCC. Thus, this study was performed to investigate the clinical significance of moesin and its biological functions in HCC. The results showed that moesin was significantly up-regulated in HCC tissues and was an independent prognostic factor for predicting the recurrence of HCC patients, postoperatively. Furthermore, we also demonstrated that moesin promoted the migration and invasion of HCC cells in vitro and in vivo. And the mechanism studies indicated that moesin overexpression increased the formation of invadopodia and improved the activation of β-catenin/MMP9 axis. Taken together, our findings revealed that moesin acted as an important onco-protein participating in the metastasis of HCC.

Keywords: Hepatocellular carcinoma; Invadopodia; Metastasis; Moesin; Recurrence; β-catenin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aged
Animals
Carcinoma, Hepatocellular / genetics*
Carcinoma, Hepatocellular / metabolism
Carcinoma, Hepatocellular / mortality
Carcinoma, Hepatocellular / pathology
Cell Line, Tumor
Cell Movement
Cell Proliferation
Female
Gene Expression Regulation, Neoplastic*
Humans
Liver Neoplasms / genetics*
Liver Neoplasms / metabolism
Liver Neoplasms / mortality
Liver Neoplasms / pathology
Lymphatic Metastasis
Male
Matrix Metalloproteinase 9 / genetics*
Matrix Metalloproteinase 9 / metabolism
Mice
Mice, Inbred NOD
Middle Aged
Neoplasm Invasiveness
Neoplasm Recurrence, Local / genetics*
Neoplasm Recurrence, Local / metabolism
Neoplasm Recurrence, Local / mortality
Neoplasm Recurrence, Local / pathology
Podosomes / metabolism
Podosomes / pathology
Podosomes / ultrastructure
Signal Transduction
Survival Analysis
Tumor Burden
beta Catenin / genetics*
beta Catenin / metabolism

Substances

CTNNB1 protein, human
beta Catenin
MMP9 protein, human
Matrix Metalloproteinase 9