Simultaneous multiplane imaging with reverberation two-photon microscopy

Nat Methods. 2020 Mar;17(3):283-286. doi: 10.1038/s41592-019-0728-9. Epub 2020 Feb 10.

Abstract

Multiphoton microscopy has gained enormous popularity because of its unique capacity to provide high-resolution images from deep within scattering tissue. Here, we demonstrate video-rate multiplane imaging with two-photon microscopy by performing near-instantaneous axial scanning while maintaining three-dimensional micrometer-scale resolution. Our technique, termed reverberation microscopy, enables the monitoring of neuronal populations over large depth ranges and can be implemented as a simple add-on to a conventional design.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acoustics
  • Animals
  • Brain / diagnostic imaging*
  • Female
  • Imaging, Three-Dimensional
  • Mice
  • Mice, Inbred C57BL
  • Microscopy, Fluorescence, Multiphoton / methods*
  • Motor Cortex / diagnostic imaging*
  • Neurons / physiology*
  • Olfactory Bulb / diagnostic imaging*
  • Optics and Photonics
  • Phantoms, Imaging
  • Photons
  • Scattering, Radiation
  • Signal-To-Noise Ratio