Development of a mesoporous silica based solid-phase extraction and ultra-performance liquid chromatography-MS/MS method for quantifying lignans in Justicia procumbens

Electrophoresis. 2020 Mar;41(5-6):379-385. doi: 10.1002/elps.201900401.

Abstract

Justicia procumbens is a food and medicine homologous variety, popularly used for making vegetable soups. In this study, a novel mesoporous silica was synthesized and used as the sorbent of SPE for the purification of lignans from J. procumbens. A laboratory-made SPE cartridge was packed with 100 mg of mesoporous silica, which was washed with 10% methanol and eluted using 0.8 mL acetonitrile after sample loading. Afterward, the extract was analyzed by ultra-performance liquid chromatography (UPLC) and MS/MS. All the lignans were efficiently separated in 6 min with the noise level in the range of 50-150 cps. 6'-Hydroxy justicidin B, 6'-hydroxy justicidin A, justicidin B, chinensinaphthol methyl ether, justicidin C, and neojusticdin A were identified to be the dominant molecular species in J. procumbens with contents of 0.065-0.37 mg/g in three tested sample batches from different geographic origins. In conclusion, the proposed mesoporous silica based SPE UPLC-MS/MS method is efficient in linearity (R2 = 0.9989-0.9996), sensitivity (LOD ≤0.13 μg/kg and LOQ ≤0.42 μg/kg), precision (RSDintra-day ≤3.12 and RSDinter-day ≤4.56), and recovery (83.42-96.11%, RSD ≤2.88%).

Keywords: Justicia procumbens; SPE; UPLC-MS/MS; lignans; mesoporous silica KCC-1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Justicia / chemistry*
  • Lignans / analysis*
  • Linear Models
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Silicon Dioxide / chemistry
  • Solid Phase Extraction / methods*
  • Tandem Mass Spectrometry / methods*

Substances

  • Lignans
  • Silicon Dioxide