The Clinical Significance of Soluble Programmed Cell Death-Ligand 1 (sPD-L1) in Patients With Gliomas

Shujun Liu; Yadi Zhu; Chenxi Zhang; Xiangrui Meng; Bo Sun; Guojun Zhang; Yubo Fan; Xixiong Kang

doi:10.3389/fonc.2020.00009

The Clinical Significance of Soluble Programmed Cell Death-Ligand 1 (sPD-L1) in Patients With Gliomas

Front Oncol. 2020 Jan 23:10:9. doi: 10.3389/fonc.2020.00009. eCollection 2020.

Authors

Shujun Liu^{1

2}, Yadi Zhu^{1

2}, Chenxi Zhang^{1

2}, Xiangrui Meng^{1

2}, Bo Sun³, Guojun Zhang^{1

2}, Yubo Fan³, Xixiong Kang^{1

2

3}

Affiliations

¹ Laboratory Diagnosis Center, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
² Beijing Engineering Research Center of Immunological Reagents and Clinical Research, Beijing, China.
³ Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University, Beijing, China.

Abstract

Background: Soluble PD-L1 (sPD-L1) in the circulation has been documented to activate global immunosuppression and is considered a predictor of negative clinical outcomes in several malignances. However, the clinical significance of sPD-L1 in the peripheral blood and cerebrospinal fluid (CSF) of patients with glioma remains unclear. Objective: The aim of this study was to detect the correlations of sPD-L1 with clinical features in brain tumors and assess the diagnostic value of this protein in gliomas. Methods: Serum samples were obtained from 73 patients with glioma, 20 patients with meningioma, and 49 healthy controls (HCs) in this study. In total, 31 CSF samples were collected from the matched glioma patients, and seven samples were collected from the matched meningioma patients. The expression of serum sPD-L1 in the glioma cohort was followed for 20 days after surgery to examine the kinetics in the circulation. Inflammatory markers were evaluated based on preoperative blood parameters. The sPD-L1 levels in the serum and CSF were determined by enzyme-linked immunosorbent assay (ELISA). The logistic regression model was used to assess the independent associations of sPD-L1 with gliomas, including high-grade gliomas. Results: Serum and CSF levels of sPD-L1 were significantly elevated in patients with gliomas compared to those with meningiomas and HCs. Additionally, increased levels of sPD-L1 were observed in relatively advanced tumors. sPD-L1 overexpression in the CSF appears to be more representative of aggressive tumor features than overexpression in the serum. For glioma diagnosis, both serum and CSF sPD-L1 showed significant value in the diagnosis and stratification of glioma, and the best diagnostic performance was obtained with serum sPD-L1 rather than blood-based inflammatory markers. In addition, a descending trend in the level of serum sPD-L1 was observed in postoperative patients. Conclusion: In gliomas, elevated circulating and CSF sPD-L1 levels are associated with aggressive biological activities. The results of the current study suggest that sPD-L1 is a promising biomarker for gliomas that can be used in clinical practice.

Keywords: biomarker; cerebrospinal fluid; glioma; inflammatory markers; soluble programmed cell death-ligand 1.