Ultrashort ssDNA in Retinoblastoma Patients Blood Plasma Detected by a Novel High Resolution HPLC Technique: a Preliminary Report

Kirill V Ermakov; Alexander A Bukhvostov; Alexander S Vedenkin; Sergey V Stovbun; Anton S Dvornikov; Dmitry A Kuznetsov

doi:10.14712/18059694.2020.8

Ultrashort ssDNA in Retinoblastoma Patients Blood Plasma Detected by a Novel High Resolution HPLC Technique: a Preliminary Report

Acta Medica (Hradec Kralove). 2019;62(4):170-173. doi: 10.14712/18059694.2020.8.

Authors

Kirill V Ermakov¹, Alexander A Bukhvostov², Alexander S Vedenkin³, Sergey V Stovbun³, Anton S Dvornikov², Dmitry A Kuznetsov^{2

3}

Affiliations

¹ N.I. Pirogov Russian National Research Medical University, Russian Federal Ministry of Health, Ostrovityanov St., 1, Moscow 117997, Russia. ermakovkv07@gmail.com.
² N.I. Pirogov Russian National Research Medical University, Russian Federal Ministry of Health, Ostrovityanov St., 1, Moscow 117997, Russia.
³ N.N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Kosygin St., 4, Moscow 119991, Russia.

PMID: 32036851
DOI: 10.14712/18059694.2020.8

Abstract

A significant population of ultrashort (50-150n) single-stranded DNA fragments were found in exosome-free blood plasma of retinoblastoma patients (6.84 ng mL-1), but not in plasma of healthy donors. An original high resolution HPLC technique has been proposed to reveal and characterize this peculiarity. To solve this task, a novel molecular size exclusion - anion exchange analytical technique was developed. Its applicability to diagnostics and oncogenesis research is quizzed here.

Keywords: HPLC; Retinoblastoma; blood plasma; cfDNA; ssDNA.

MeSH terms

Adult
Biomarkers, Tumor / genetics*
Case-Control Studies
Child, Preschool
Chromatography, High Pressure Liquid / methods*
DNA, Single-Stranded*
Humans
Male
Retinal Neoplasms / diagnosis*
Retinal Neoplasms / genetics*
Retinoblastoma / diagnosis*
Retinoblastoma / genetics*

Substances

Biomarkers, Tumor
DNA, Single-Stranded