Conjugation of proteins to AMP (AMPylation) is a prevalent post-translational modification (PTM) in human cells, involved in the regulation of unfolded protein response and neural development. Here we present a tailored pronucleotide probe suitable for in situ imaging and chemical proteomics profiling of AMPylated proteins. Using straightforward strain-promoted azide-alkyne click chemistry, the probe provides stable fluorescence labelling in living cells.
Keywords: AMPylation; click chemistry; fluorescent probes; post-translational modifications; proteomics.
© 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.