A Pronucleotide Probe for Live-Cell Imaging of Protein AMPylation

Chembiochem. 2020 May 4;21(9):1285-1287. doi: 10.1002/cbic.201900716. Epub 2020 Feb 6.

Abstract

Conjugation of proteins to AMP (AMPylation) is a prevalent post-translational modification (PTM) in human cells, involved in the regulation of unfolded protein response and neural development. Here we present a tailored pronucleotide probe suitable for in situ imaging and chemical proteomics profiling of AMPylated proteins. Using straightforward strain-promoted azide-alkyne click chemistry, the probe provides stable fluorescence labelling in living cells.

Keywords: AMPylation; click chemistry; fluorescent probes; post-translational modifications; proteomics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Monophosphate / chemistry*
  • Alkynes / chemistry
  • Azides / chemistry
  • Click Chemistry
  • Fluorescence
  • HeLa Cells
  • Humans
  • Molecular Imaging
  • Protein Processing, Post-Translational*
  • Proteins / chemistry*
  • Proteins / metabolism
  • Proteome / analysis
  • Proteome / metabolism*

Substances

  • Alkynes
  • Azides
  • Proteins
  • Proteome
  • Adenosine Monophosphate