The complete genome of Banana streak GF virus Yunnan isolate infecting Cavendish Musa AAA group in China

Wei-Li Li; Nai-Tong Yu; Jian-Hua Wang; Jun-Cheng Li; Zhi-Xin Liu

doi:10.7717/peerj.8459

The complete genome of Banana streak GF virus Yunnan isolate infecting Cavendish Musa AAA group in China

PeerJ. 2020 Jan 27:8:e8459. doi: 10.7717/peerj.8459. eCollection 2020.

Authors

Wei-Li Li^#¹, Nai-Tong Yu^#^{1

2}, Jian-Hua Wang¹, Jun-Cheng Li³, Zhi-Xin Liu^{1

2}

Affiliations

¹ Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
² Hainan Key Laboratory of Tropical Microbe Resources, Haikou, China.
³ Guangdong Key Laboratory of Tropical and Subtropical Fruit Tree Research, Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China.

^# Contributed equally.

Abstract

Banana streak virus (BSV) belongs to the members of the genus Badnavirus, family Caulimoviridae. At present, BSV contains nine species in the International Committee on Taxonomy of Viruses (ICTV) classification report (2018b release). Previous study indicated that the viral particles of Banana streak virus Acuminata Yunnan (BSV-Acum) were purified from banana (Cavendish Musa AAA group) leaves in Yunnan Province, China, and its complete genome was obtained. To further determine whether this sample infecting with Banana streak GF virus (BSGFV), the polymerase chain reaction (PCR) cloning and complete genome analysis of the Banana streak GF virus Yunnan isolate (BSGFV-YN) isolate were carried out in this study. The result showed that BSGFV-YN infecting Cavendish Musa AAA group was co-infecting this sample. Its genome contains a total of 7,325 bp in length with 42% GC content. This complete genome sequence was deposited in GenBank under accession number MN296502. Sequence analysis showed that the complete genome of BSGFV-YN was 98.14% sequence similarity to BSGFV Goldfinger, while it was 49.10-57.09% to other BSV species. Two phylogenetic trees based on the complete genome and ORFIII polyprotein indicated that BSGFV-YN and other BSV species clustered into a group, while it was the highest homology with BSGFV Goldfinger. Although BSGFV-YN and BSGFV Goldfinger were highly homologous, their cultivating bananas are different. The former cultivating banana was from Cavendish Musa AAA group, while the latter cultivating banana was from Goldfinger Musa AAAB group. Compared with BSGFV Goldfinger, the genome of BSGFV-YN has an extra multiple repetitive sequences in the intergenetic region between ORFIII and ORFI, suggesting that this region might be related to host selection. In summary, a BSGFV-YN distant from BSV-Acum was identified from the same sample, and its complete genome sequence was determined and analyzed. The study extends the polymorphism of BSVs in China and provides scientific clue for the evolutionary relationship with host selection of badnaviruses.

Keywords: Badnavirus; Banana streak GF virus; Musa spp; Viral genome.

Grants and funding

This study was funded by the Young Elite Scientists Sponsorship Program by CSTC (Project No. CSTC-QN201704) and Guangdong Key Laboratory of Tropical and Subtropical Fruit Tree Research, Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences (No. 2017B030314113). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.