Enhanced production of questin by marine-derived Aspergillus flavipes HN4-13

Lei Guo; Le Wang; Xiangrong Li; Xiaowen Xu; Jiacai Guo; Xintong Wang; Weiqin Yang; Fuxuan Xu; Fuhou Li

doi:10.1007/s13205-020-2067-z

Enhanced production of questin by marine-derived Aspergillus flavipes HN4-13

3 Biotech. 2020 Feb;10(2):54. doi: 10.1007/s13205-020-2067-z. Epub 2020 Jan 21.

Authors

Lei Guo^{1

2

3}, Le Wang^{1

2}, Xiangrong Li^{1

2}, Xiaowen Xu^{1

2}, Jiacai Guo^{1

2}, Xintong Wang^{1

2}, Weiqin Yang^{1

2}, Fuxuan Xu^{1

2

3}, Fuhou Li^{1

2

3}

Affiliations

¹ Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, 222005 China.
² Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, 222005 China.
³ Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, 222005 China.

Abstract

Questin has favorable applications. Fractional factorial design, Box-Behnken design, and response surface methodology were adopted to optimize the fermentation conditions of the marine-derived fungus, Aspergillus flavipes HN4-13, thereby enhancing questin production. Optimal fermentation conditions in a 500-mL conical flask with 200 mL of medium were 4% soluble starch, 0.9% beef extract, 4% NaCl, 0.05% Na₂HPO₄, pH 6, 2% inoculum size, and shaking at 28 ℃ and 160 rpm/min for 7 days. The production of questin can achieve 64.93 ± 4.55 mg/L, with no significant difference from the predicted value (66.27 mg/L). Thus, this optimized process of questin production is feasible. Such production is 17-fold higher than that of the basal Sabouraud's dextrose medium. Results indicate the potential of A. flavipes HN4-13 in the large-scale production of questin through fermentation.

Keywords: Aspergillus flavipes HN4-13; Fermentation optimization; Questin; Response surface methodology.

Publication types

Case Reports