Comparison of MIC Test Strip and Sensititre YeastOne with the CLSI and EUCAST Broth Microdilution Reference Methods for In Vitro Antifungal Susceptibility Testing of Cryptococcus neoformans

Antimicrob Agents Chemother. 2020 Mar 24;64(4):e02261-19. doi: 10.1128/AAC.02261-19. Print 2020 Mar 24.

Abstract

We compared MIC test strip (MTS) and Sensititre YeastOne (SYO) methods with EUCAST and CLSI methods for amphotericin B, 5-fluocytosine, fluconazole, voriconazole, and isavuconazole against 106 Cryptococcus neoformans isolates. The overall essential agreement between the EUCAST and CLSI methods was >72% and >94% at ±1 and ±2 dilutions, respectively. The essential agreements between SYO and EUCAST/CLSI for amphotericin B, 5-flucytosine, fluconazole, and voriconazole were >89/>93% and between MTS and EUCAST/CLSI were >57/>75%. Very major error rates were low for amphotericin B and fluconazole (<3%) and a bit higher for the other drugs (<8%).

Keywords: CLSI; Cryptococcus neoformans; EUCAST; Liofilchem MIC test strip; Sensititre YeastOne; antifungal susceptibility.

Publication types

  • Comparative Study

MeSH terms

  • Antifungal Agents / pharmacology*
  • Cryptococcosis / microbiology
  • Cryptococcus neoformans / drug effects*
  • Cryptococcus neoformans / isolation & purification
  • Humans
  • Microbial Sensitivity Tests / methods*
  • Microbial Sensitivity Tests / standards
  • Time Factors

Substances

  • Antifungal Agents