Transglutaminase orthologues in medaka fish - biochemical characterization and establishment of gene-deficient mutants

Anal Biochem. 2020 Sep 1:604:113610. doi: 10.1016/j.ab.2020.113610. Epub 2020 Jan 31.

Abstract

By genome analysis, seven homologous genes (orthologues) of human transglutaminases (TGases) have been identified in medaka fish (Oryzias latipes), some of which clearly corresponded to Factor XIII, TG1, and TG2. The enzymatically active-recombinant proteins for these medaka TGases have been successfully produced in bacteria or baculovirus-infected insect cell systems. Specific antibodies have been prepared and used in immunohistochemical analyses to reveal tissue distribution. Furthermore, gene-deficient medaka mutants for the genes encoding Factor XIII and TG1 have been established together with analysis of their phenotypes. Retarded cross-linking of fibrin and higher sensitivity to osmolality are observed when each gene is knocked-out. In this review, we summarize these biochemical features and the phenotypes of these gene-deficient fish.

Keywords: Genome editing; Immunohistochemistry; Medaka; Transglutaminase.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Fish Proteins / genetics
  • Fish Proteins / metabolism*
  • Gene Knockout Techniques
  • Oryzias / genetics*
  • Oryzias / metabolism
  • Phenotype
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Transglutaminases / genetics
  • Transglutaminases / metabolism*

Substances

  • Fish Proteins
  • Recombinant Proteins
  • Transglutaminases