Characterization of microbial community and resistance gene (CzcA) shifts in up-flow constructed wetlands-microbial fuel cell treating Zn (II) contaminated wastewater

Qian Wang; Ruiyuan Lv; Eldon R Rene; Xiaoyu Qi; Qiang Hao; Yuanda Du; Congcong Zhao; Fei Xu; Qiang Kong

doi:10.1016/j.biortech.2020.122867

Characterization of microbial community and resistance gene (CzcA) shifts in up-flow constructed wetlands-microbial fuel cell treating Zn (II) contaminated wastewater

Bioresour Technol. 2020 Apr:302:122867. doi: 10.1016/j.biortech.2020.122867. Epub 2020 Jan 23.

Authors

Qian Wang¹, Ruiyuan Lv², Eldon R Rene³, Xiaoyu Qi¹, Qiang Hao⁴, Yuanda Du¹, Congcong Zhao¹, Fei Xu¹, Qiang Kong⁵

Affiliations

¹ College of Geography and Environment, Collaborative Innovation Center of Human-Nature and Green Development in the Universities of Shandong, Shandong Normal University, Jinan 250014, PR China.
² College of Geography and Environment, Collaborative Innovation Center of Human-Nature and Green Development in the Universities of Shandong, Shandong Normal University, Jinan 250014, PR China; Institute of Environment and Ecology, Shandong Normal University, Jinan 250014, PR China.
³ Department of Environmental Engineering and Water Technology, IHE Delft Institute for Water Education, Westvest 7, 2611 AX Delft, The Netherlands.
⁴ Center for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo, Sydney, NSW, Australia.
⁵ College of Geography and Environment, Collaborative Innovation Center of Human-Nature and Green Development in the Universities of Shandong, Shandong Normal University, Jinan 250014, PR China; Department of Civil and Environmental Engineering, National University of Singapore, Singapore 117576, Singapore. Electronic address: kongqiang@sdnu.edu.cn.

PMID: 32007853
DOI: 10.1016/j.biortech.2020.122867

Abstract

The main aim of this work was to characterize the microbial community structure and resistance gene (CzcA) shifts in up-flow constructed wetlands-microbial fuel cell (CW-MFC) treating Zn (II) contaminated wastewater. Two CW-MFC devices were operated, i.e. the experimental group (EG) treating Zn (II) wastewater, and the control group (CG) treating Zn (II)-free wastewater. The results showed the CW-MFC combination exhibited good removal efficiency on Zn (II), while the average voltage, the power density and the removal rates (TP, TN, NH₄⁺-N and COD) significantly reduced (p < 0.05). The microbial community structure showed that the Zn (II) significantly reduced the abundance of some functional genus (p < 0.05), such as Ochrobactrum, Nitrosomonas, Pseudomonas and Dechloromonas. Zn (II) inhibited the microbial richness in the anode, but it played a positive role in the cathode. Anew, the expression of the CzcA in the CW-MFC was promoted by Zn (II), particularly in the cathode.

Keywords: Bioelectricity; Constructed wetlands; CzcA gene; Microbial fuel cell; Zn (II) contamination.

MeSH terms

Bioelectric Energy Sources*
Electricity
Electrodes
Microbiota*
Wastewater
Wetlands
Zinc

Substances

Waste Water
Zinc