Influence of seasonal variation on phenolic content and in vitro antioxidant activity of Secondatia floribunda A. DC. (Apocynaceae)

Daiany Alves Ribeiro; Cicera Janaine Camilo; Carla de Fátima Alves Nonato; Fabiola Fernandes Galvão Rodrigues; Irwin Rose Alencar Menezes; Jaime Ribeiro-Filho; Jianbo Xiao; Marta Maria de Almeida Souza; José Galberto Martins da Costa

doi:10.1016/j.foodchem.2020.126277

Influence of seasonal variation on phenolic content and in vitro antioxidant activity of Secondatia floribunda A. DC. (Apocynaceae)

Food Chem. 2020 Jun 15:315:126277. doi: 10.1016/j.foodchem.2020.126277. Epub 2020 Jan 22.

Authors

Daiany Alves Ribeiro¹, Cicera Janaine Camilo¹, Carla de Fátima Alves Nonato², Fabiola Fernandes Galvão Rodrigues³, Irwin Rose Alencar Menezes⁴, Jaime Ribeiro-Filho⁵, Jianbo Xiao⁶, Marta Maria de Almeida Souza⁷, José Galberto Martins da Costa⁸

Affiliations

¹ Programa de Pós-Graduação em Etnobiologia e Conservação da Natureza, Universidade Regional do Cariri, Crato, CE, Brazil; Laboratório de Pesquisas de Produtos Naturais, Universidade Regional do Cariri, Crato, CE, Brazil.
² Laboratório de Pesquisas de Produtos Naturais, Universidade Regional do Cariri, Crato, CE, Brazil.
³ Laboratório de Pesquisas de Produtos Naturais, Universidade Regional do Cariri, Crato, CE, Brazil; Programa de Pós-Graduação em Ensino em Saúde, Centro Universitário Dr. Leão Sampaio, Juazeiro do Norte, CE, Brazil.
⁴ Laboratório de Farmacologia e Química Molecular, Universidade Regional do Cariri, Crato, CE, Brazil.
⁵ Instituto Gonçalo Moniz, Fundação Oswaldo Cruz (Fiocruz-BA), Salvador, Bahia, Brazil.
⁶ Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Taipa, Macau.
⁷ Laboratório de Ecologia Vegetal, Universidade Regional do Cariri, Crato, CE, Brazil.
⁸ Programa de Pós-Graduação em Etnobiologia e Conservação da Natureza, Universidade Regional do Cariri, Crato, CE, Brazil; Laboratório de Pesquisas de Produtos Naturais, Universidade Regional do Cariri, Crato, CE, Brazil; Programa de Pós-Graduação em Ensino em Saúde, Centro Universitário Dr. Leão Sampaio, Juazeiro do Norte, CE, Brazil. Electronic address: galberto.martins@urca.br.

PMID: 32004983
DOI: 10.1016/j.foodchem.2020.126277

Abstract

This study reports the effects of seasonal variation on the total polyphenol and flavonoid content and the in vitro antioxidant activity of Secondatia floribunda A. DC. The extracts were prepared from the inner bark and heartwood of samples harvested in the 2015 to 2016. The total phenolic and flavonoid content was determined by specific qualitative tests. The in vitro antioxidant capacity was analyzed using the following tests: 1-1-diphenyl-2-picrylhydrazyl radical (DPPH), 2,2'-azinobis-3-ethylbenzenothiazoline-6-sulfonic acid (ABTS⁺), ferric reducing antioxidant power (FRAP) and iron (Fe²⁺) chelating activity. The total polyphenol and total flavonoid content varied over the harvest period. The DPPH and ABTS tests revealed that in the dry season had the most potent in vitro antioxidant activity, although the extracts obtained during the rainy season presented the higher Fe²⁺ chelating and Ferric reducing activities. In conclusion, the phenolic content and in vitro antioxidant activity are correlated, and both are influenced by seasonality.

Keywords: Chemical ecology; In vitro antioxidant activity; Phenolic compounds; Seasonality; Secondatia floribunda.

MeSH terms

Antioxidants / analysis*
Apocynaceae / chemistry*
Flavonoids / chemistry
Plant Extracts / chemistry
Polyphenols / analysis*
Seasons

Substances

Antioxidants
Flavonoids
Plant Extracts
Polyphenols