Application of condensed molasses fermentation solubles and lactic acid bacteria in corn silage production

J Sci Food Agric. 2020 Apr;100(6):2722-2731. doi: 10.1002/jsfa.10304. Epub 2020 Feb 17.

Abstract

Background: The aim of this study is to investigate the application of two lactic acid bacteria and dry condensed molasses fermentation solubles (CMS) in the making and preservation of corn silage. Forage corn materials are divided into eight treatment groups as follows: (i) control, (ii) B2 (Lactobacillus plantarum B2, 1 × 109 cfu kg-1 ), (iii) LAS (Lactobacillus buchneri 40788, 3 × 108 cfu kg-1 ), (iv) B2 + LAS, (v) CMS (35 g kg-1 , fresh weight), (vi) B2 + CMS, (vii) LAS + CMS and (viii) B2 + LAS + CMS. The silage composition and aerobic stability are determined after ensiling for 90 days. Furthermore, the digestibility of the silage product and gas production are evaluated using a trotro digestion procedure.

Results: The assay results indicate that the CMS supplementation and B2 inoculation significantly increased lactic acid concentration (P < 0.01). However, they also reduced the content of acetic acid and silage pH at the initial fermentation stage. The CMS supplemented with B2 (B2 + CMS) showed an improvement in the quality of silage, but a significant decrease in aerobic stability (P < 0.01). The B2 + LAS + CMS treatment yielded an increase in acetic acid production during the late fermentation period and is able to extend the aerobic stability of corn silage. Furthermore, this study shows that CMS supplementation alone can significantly improve the digestibility of the in vitro dry matter (P < 0.01) and the microbial protein synthesis efficiency (P = 0.01). In addition, the CMS supplementation is beneficial for enhancing the aerobic stability of corn silage.

Conclusions: These results suggest that the combination of CMS supplementation and a suitable inoculation lactic acid bacterial strain can be highly promising for enhancing the higher quality and stability of corn silage. © 2020 Society of Chemical Industry.

Keywords: aerobic stability; condensed molasses fermentation solubles (CMS); corn silage; lactic acid bacteria (LAB).

MeSH terms

  • Fermentation
  • Lactobacillus / physiology*
  • Lactobacillus plantarum / physiology*
  • Molasses
  • Silage / analysis*
  • Silage / microbiology*
  • Zea mays / chemistry
  • Zea mays / microbiology

Supplementary concepts

  • Lactobacillus buchneri