Interventional effects of the direct application of "Sanse powder" on knee osteoarthritis in rats as determined from lipidomics via UPLC-Q-Exactive Orbitrap MS

Peng Wu; Zhengquan Huang; Jinjun Shan; Zichen Luo; Nongshan Zhang; Songjiang Yin; Cunsi Shen; Runlin Xing; Wei Mei; Yancheng Xiao; Bo Xu; Jun Mao; Peimin Wang

doi:10.1186/s13020-020-0290-5

Interventional effects of the direct application of "Sanse powder" on knee osteoarthritis in rats as determined from lipidomics via UPLC-Q-Exactive Orbitrap MS

Chin Med. 2020 Jan 23:15:9. doi: 10.1186/s13020-020-0290-5. eCollection 2020.

Authors

Peng Wu^#^{1

2

3}, Zhengquan Huang^#^{1

2}, Jinjun Shan^{4

5}, Zichen Luo^{4

5}, Nongshan Zhang^{1

2}, Songjiang Yin^{1

2}, Cunsi Shen^{4

5}, Runlin Xing^{1

2}, Wei Mei^{1

2}, Yancheng Xiao^{1

2}, Bo Xu^{1

2}, Jun Mao^{1

2}, Peimin Wang^{1

2}

Affiliations

¹ 1Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029 China.
² 2Jiangsu Province Hospital of Chinese Medicine, Nanjing, 210029 China.
³ 5Key Laboratory for Metabolic Diseases in Chinese Medicine, First College of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023 China.
⁴ 3Medical Metabolomics Center, Nanjing University of Chinese Medicine, Nanjing, 210023 China.
⁵ 4Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing, 210023 China.

^# Contributed equally.

Abstract

Background: Our previous clinical evidence suggested that the direct application of "Sanse powder" the main ingredient of "Yiceng" might represent an alternative treatment for knee osteoarthritis. However, the mechanism underlying its effect is poorly understood. In this study, we investigated the mechanism of the effect of direct "Sanse powder" application for the treatment of knee osteoarthritis (KOA) in rats by using lipidomics.

Methods: KOA rats were established by cutting the anterior cruciate ligament, and the cold pain threshold and mechanical withdrawal threshold (MWT) of seven rats from each group were measured before modelling (0 days) and at 7, 14, 21 and 28 days after modelling. Histopathological evaluation of the synovial tissue was performed by haematoxylin and eosin (H&E) staining after modelling for 28 days. Interleukin-1β (IL-1β), pro-interleukin-1β (pro-IL-1β) and tumor necrosis factor-α (TNF-α) proteins in synovial tissue were measured by western blot, and the mRNA expression levels of IL-1β and TNF-α in synovial tissue were measured using Real-time reverse transcription polymerase chain reaction (qRT-PCR), the levels of IL-1β and TNF-α in rat serum were measured by enzyme-linked immunosorbent assay (ELISA), Serum lipid profiles were obtained by using ultra-performance liquid chromatography combined with quadrupole-Exactive Orbitrap mass spectrometry (UPLC-Q-Exactive Orbitrap MS).

Results: The results confirmed that the direct application of "Sanse powder" had a significant protective effect against KOA in rats. Treatment with "Sanse powder" not only attenuated synovial tissue inflammation but also increased the levels of the cold pain threshold and MWT. In addition, the lipidomics results showed that the levels of diacylglycerol (DAG), triacylglycerols (TAGs), lysophosphatidylcholine (LPC), phosphatidylcholine (PC), fatty acid esters of hydroxy fatty acids (FAHFAs), and phosphatidylethanolamine (PE) were restored almost to control levels following treatment.

Conclusions: Lipidomics provides a better understanding of the actions of direct application "Sanse powder" therapy for KOA.

Keywords: Direct application “Sanse powder” therapy; KOA lipidomics; LC–MS; Prescription.