Why not add some SPARKL to your life (and death)!?

Jesse D Gelles; Jerry Edward Chipuk

doi:10.1080/23723556.2019.1685841

Why not add some SPARKL to your life (and death)!?

Mol Cell Oncol. 2019 Nov 17;7(1):1685841. doi: 10.1080/23723556.2019.1685841. eCollection 2020.

Authors

Jesse D Gelles^{1

2}, Jerry Edward Chipuk^{1

2

3

4

5}

Affiliations

¹ Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
² The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
³ Department of Dermatology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
⁴ The Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
⁵ The Diabetes, Obesity, and Metabolism Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

Abstract

Quantifying cytostatic and cytotoxic outcomes of cells responding to perturbagens is an essential component of mechanism-based studies and pharmacological screening approaches. We recently described an easy and versatile method for single-cell and population-level analyses using real-time kinetic labeling (SPARKL). This technology enables zero-handling, non-disruptive protocols for integrating proliferation profiles with cell death mechanisms, along with advanced mathematics for robust analyses.

Keywords: Apoptosis; SPARKL; cell death; ferroptosis; high-throughput; kinetics; live-cell imaging; necroptosis; proliferation; real-time analysis.

Abstract

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