The radiation safety culture of those working in university open-source radioactive material laboratories was assessed by conducting both surveys and behavioral observations. Baseline results (n = 82, 89% response rate) of assessed safety culture categories indicated safety practices and safety compliance were the most in need of improvement. Specific training based on these results was provided to laboratory members at Princeton University, with creative signage and a safety newsletter posted in and around laboratories for reinforcement, in a targeted effort to improve the radiation safety culture. Signage posted utilized pop cultural memes and other engaging graphics designed to raise awareness of appropriate safety practices and the minimum laboratory attire expected while working in radioactive material laboratories. Postintervention results (n = 38, 43% response rate) indicated improvement in 4 out of the 10 safety culture indicators considered as well as fewer instances of improper safety practices. Collaborative techniques and increased communication between researchers and radiation safety staff appear to have initiated an improvement in the radiation safety culture in open-source radioactive material laboratories at Princeton University.