Transcriptomics integrated with metabolomics reveals the effect of regulated deficit irrigation on anthocyanin biosynthesis in Cabernet Sauvignon grape berries

Bohan Yang; Shuang He; Yuan Liu; Buchun Liu; Yanlun Ju; Dengzhao Kang; Xiangyu Sun; Yulin Fang

doi:10.1016/j.foodchem.2020.126170

Transcriptomics integrated with metabolomics reveals the effect of regulated deficit irrigation on anthocyanin biosynthesis in Cabernet Sauvignon grape berries

Food Chem. 2020 Jun 1:314:126170. doi: 10.1016/j.foodchem.2020.126170. Epub 2020 Jan 13.

Authors

Bohan Yang¹, Shuang He¹, Yuan Liu², Buchun Liu², Yanlun Ju¹, Dengzhao Kang³, Xiangyu Sun⁴, Yulin Fang⁵

Affiliations

¹ College of Enology, College of Food Science and Engineering, Viti-viniculture Engineering Technology Center of State Forestry and Grassland Administration, Shaanxi Engineering Research Center for Viti-Viniculture, Heyang Viti-viniculture Station, Northwest A&F University, Yangling 712100, China.
² Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
³ College of Enology, College of Food Science and Engineering, Viti-viniculture Engineering Technology Center of State Forestry and Grassland Administration, Shaanxi Engineering Research Center for Viti-Viniculture, Heyang Viti-viniculture Station, Northwest A&F University, Yangling 712100, China; Xinjiang Panyu Winery Co. LTD, Bohu 841400, China.
⁴ College of Enology, College of Food Science and Engineering, Viti-viniculture Engineering Technology Center of State Forestry and Grassland Administration, Shaanxi Engineering Research Center for Viti-Viniculture, Heyang Viti-viniculture Station, Northwest A&F University, Yangling 712100, China. Electronic address: sunxiangyu@nwafu.edu.cn.
⁵ College of Enology, College of Food Science and Engineering, Viti-viniculture Engineering Technology Center of State Forestry and Grassland Administration, Shaanxi Engineering Research Center for Viti-Viniculture, Heyang Viti-viniculture Station, Northwest A&F University, Yangling 712100, China. Electronic address: fangyulin@nwsuaf.edu.cn.

PMID: 31978717
DOI: 10.1016/j.foodchem.2020.126170

Abstract

Regulated deficit irrigation (RDI) is a new type of water-saving irrigation technology developed in recent years which was well suited to arid and semi-arid grape plant areas. The anthocyanin synthesis of grapes under RDI was revealed through omics in this study. RDI slightly decreased the hundred-grain weight and increased the soluble solid content, juice pH, reducing sugar content, and total anthocyanin content. Meanwhile, the total acid content decreased before ripening. Transcriptomics and metabolomics analyses revealed that large numbers of differentially expressed genes (DEGs) and significantly changed metabolites (SCMs) were filtered in the RDI groups. RDI1 with 30% ETc upregulated 7 related gene expression levels in the anthocyanin biosynthetic pathway and also increased some metabolites contents. Eventually, the contents of most monomeric anthocyanins in the RDI groups were increased, and the proportion of Mv increased in the ripe grapes of the RDI groups. In all, RDI is a useful water-saving irrigation method which could also increase anthocyanin content in grapes.

Keywords: Anthocyanin biosynthesis; Grape; Metabolomics; Regulated deficit irrigation; Transcriptomics.

MeSH terms

Anthocyanins / analysis*
Anthocyanins / biosynthesis
Fruit / chemistry
Metabolomics*
Transcriptome*
Vitis / chemistry*

Substances

Anthocyanins