Identification of Three Differentially Expressed miRNAs as Potential Biomarkers for Lung Adenocarcinoma Prognosis

Wei Wang; Bin Liu; Xiaoran Duan; Xiaolei Feng; Tuanwei Wang; Pengpeng Wang; Mingcui Ding; Qiao Zhang; Feifei Feng; Yongjun Wu; Wu Yao; Qi Wang; Yongli Yang

doi:10.2174/1386207323666200124123103

Identification of Three Differentially Expressed miRNAs as Potential Biomarkers for Lung Adenocarcinoma Prognosis

Comb Chem High Throughput Screen. 2020;23(2):148-156. doi: 10.2174/1386207323666200124123103.

Authors

Wei Wang^{1

2}, Bin Liu^{1

2}, Xiaoran Duan¹, Xiaolei Feng¹, Tuanwei Wang¹, Pengpeng Wang¹, Mingcui Ding¹, Qiao Zhang³, Feifei Feng^{2

3}, Yongjun Wu^{2

3}, Wu Yao¹, Qi Wang¹, Yongli Yang⁴

Affiliations

¹ Department of Occupational Health and Occupational Disease, College of Public Health, Zhengzhou University, Zhengzhou, China.
² The Key Laboratory of Nanomedicine and Health Inspection of Zhengzhou, Zhengzhou, China.
³ Department of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou, China.
⁴ Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, Zhengzhou, China.

PMID: 31976830
DOI: 10.2174/1386207323666200124123103

Abstract

Objective: The aim of this study areto screen MicroRNAs (miRNAs) related to the prognosis of lung adenocarcinoma (LUAD) and to explore the possible molecular mechanisms.

Methods: The data for a total of 535 patients with LUAD data were downloaded from The Cancer Genome Atlas (TCGA) database. The miRNAs for LUAD prognosis were screened by both Cox risk proportional regression model and Last Absolute Shrinkage and Selection Operator (LASSO) regression model. The performances of the models were verified by time-dependent Receiver Operating Characteristic (ROC) curve. The possible biological processes linked to the miRNAs' target genes were analyzed by Gene Ontology (GO), Kyoto gene and genome encyclopedia (KEGG).

Results: Among 127 differentially expressed miRNAs identified from the screening analysis, there are 111 up-regulated and 16 down-regulated miRNAs. Three of them, hsa-miR-1293, hsa-miR-490 and hsa-miR- 5571, were also significantly associated with the survival of the LUAD patients. The targets of the three miRNAs are significantly enriched in systemic lupus erythematosus pathways.

Conclusion: Hsa-miR-1293, hsa-miR-490 and hsa-miR-5571 can be potentially used as novel biomarkers for the prognosis prediction of LUAD.

Keywords: Lung adenocarcinoma; TCGA; biomarkers; computational approaches; miRNAs; prognosis..

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenocarcinoma of Lung / diagnosis*
Adenocarcinoma of Lung / genetics
Biomarkers, Tumor / genetics*
Humans
Lung Neoplasms / diagnosis*
Lung Neoplasms / genetics
MicroRNAs / genetics*

Substances

Biomarkers, Tumor
MIRN1293 microRNA, human
MIRN490 microRNA, human
MicroRNAs