The mitochondrial genome of Apion squamigerum (Coleoptera, Curculionoidea, Brentidae) and the phylogenetic implications

Nan Song; Xinxin Li; Xinming Yin; Xinghao Li; Shengjun Yin; Mingsheng Yang

doi:10.7717/peerj.8386

The mitochondrial genome of Apion squamigerum (Coleoptera, Curculionoidea, Brentidae) and the phylogenetic implications

PeerJ. 2020 Jan 13:8:e8386. doi: 10.7717/peerj.8386. eCollection 2020.

Authors

Nan Song¹, Xinxin Li¹, Xinming Yin¹, Xinghao Li¹, Shengjun Yin², Mingsheng Yang³

Affiliations

¹ College of Plant Protection, Henan Agricultural University, Zhengzhou, China.
² Department of Chinese Medicine, The Second Hospital of Tianjin Medical University, Tianjin, China.
³ College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, China.

Abstract

In this article, we present the nearly complete mitochondrial genome (mitogenome) of the weevil beetle Apion squamigerum (Curculionoidea, Brentidae), assembled using data from Illumina next generation sequencing (NGS). This mitogenome was found to be very large, with the total length of 18,562 bp. Two trnM genes were identified. A large non-coding intergenic spacer spanning 1,949 bp occurred between trnI and trnM2. Combined with 111 existing weevil mitogenomes, we conducted phylogenetic reconstructions based on various datasets under maximum likelihood and Bayesian inference. Firstly, phylogenetic analyses robustly supported a sister group of A. squamigerum and Rhopalapion longirostre, namely, that two species of Apioninae (Brentidae) formed a clade. Within the entire Curculionoidea, the Nemonychidae diverged firstly, following the families Anthribidae and Attelabidae. In addition, a large clade comprising the sister families Brentidae and Curculionidae was strongly supported in all trees.

Keywords: Brentid beetle; Large intergenic spacer; Mitogenome; Phylogeny.

Grants and funding

This research is supported by the Science and Technology Innovation Fund of Henan Agricultural University (KJCX2019A10), Earmarked Fund for China Agriculture Research System (NO. CARS-27), Key Scientific Research Projects of Henan Province (Grant Nos. 16A210029, 18B210006). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.