An electric soldering iron ion source (ESII) coupling with rapid evaporative ionization mass spectrometry (REIMS) was developed and used for in situ monitoring the dynamic variation trend in oxidation characteristics of fish oil during storage. The lipidomics profiles of fish oil stored at various days were acquired by ESII-REIMS. The fatty acid and triacylglycerol species were structurally identified, and their abundances were analyzed according to multivariate statistical models mainly including principle component analysis as well as orthogonal partial least-squares analysis. On the shared and unique structure plot, the ions of m/z 255.23, 281.24, 877.72, and 901.72 displayed the most significant variation among the oxidized fish oil samples. Based on receiver operating characteristic curve analysis with an optimal Youden index of 0.91, these markers were further verified. The variation of viscosity and volatiles were also evaluated to further verify the oxidation characteristics of fish oil. The study demonstrated that ESII-REIMS technology used as an advanced detection method could ensure fish oil quality during storage.
Keywords: REIMS; fish oil; lipidomics; multivariate analysis model; oxidation characteristic.