Advanced approaches for elucidating structures of large RNAs using NMR spectroscopy and complementary methods

Anita Kotar; Hannah N Foley; Kirk M Baughman; Sarah C Keane

doi:10.1016/j.ymeth.2020.01.009

Advanced approaches for elucidating structures of large RNAs using NMR spectroscopy and complementary methods

Methods. 2020 Nov 1:183:93-107. doi: 10.1016/j.ymeth.2020.01.009. Epub 2020 Jan 20.

Authors

Anita Kotar¹, Hannah N Foley², Kirk M Baughman², Sarah C Keane³

Affiliations

¹ Biophysics Program, University of Michigan, 930 N. University Ave, Ann Arbor, MI 48109-1055, United States.
² Department of Chemistry, University of Michigan, 930 N. University Ave, Ann Arbor, MI 48109-1055, United States.
³ Biophysics Program, University of Michigan, 930 N. University Ave, Ann Arbor, MI 48109-1055, United States; Department of Chemistry, University of Michigan, 930 N. University Ave, Ann Arbor, MI 48109-1055, United States. Electronic address: sckeane@umich.edu.

PMID: 31972224
DOI: 10.1016/j.ymeth.2020.01.009

Abstract

NMR spectroscopy is a key technique that has significantly advanced our understanding of RNA structure and dynamics. However, determination of large RNA structures by NMR spectroscopy remains a significant technical challenge. In this review, we highlight advances that facilitate NMR studies of large RNAs, including methods for sample preparation, isotope labeling strategies, and data acquisition. In addition, we review hybrid approaches that have been instrumental in the structure determination of large RNAs.

Keywords: Hybrid methods; Isotopic labeling; NMR spectroscopy; RNA structure; Residual dipolar coupling; Segmental labeling.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Isotope Labeling / methods*
Neutron Diffraction / methods
Nuclear Magnetic Resonance, Biomolecular / methods*
Nucleic Acid Conformation*
RNA / chemistry*
Scattering, Small Angle*
X-Ray Diffraction / methods

Substances

RNA