Identification of proteasome subunit alpha type-1 as a novel biomarker in HBV-associated hepatocellular carcinoma tissue interstitial fluid by proteomic analysis

Jian Qin; Bingshuang Long; Lanying Luo; Yi Wei; Shiyi Chen; You Li; Xue Liang; Zhiyong Zhang

Identification of proteasome subunit alpha type-1 as a novel biomarker in HBV-associated hepatocellular carcinoma tissue interstitial fluid by proteomic analysis

Int J Clin Exp Pathol. 2017 Jul 1;10(7):7812-7820. eCollection 2017.

Authors

Jian Qin¹, Bingshuang Long¹, Lanying Luo², Yi Wei¹, Shiyi Chen¹, You Li¹, Xue Liang¹, Zhiyong Zhang¹

Affiliations

¹ Department of Occupational and Environmental Health, School of Public Health, Guangxi Medical University Nanning, Guangxi, P. R. China.
² Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control Nanning, Guangxi, P. R. China.

PMID: 31966629
PMCID: PMC6965243

Abstract

Differentially expressed proteins between HCC TIF and normal interstitial fluid of adjacent nontumor tissues were identified through comparative proteomics approach. Then, two-dimensional gel electrophoresis (2-DE), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), In-Cell Western technique, and reverse transcription-polymerase chain reaction (RT-PCR) were used to verify differentially expressed proteins. As a result, through 2-DE, 69 spots were roughly recognized as differentially expressed protein spots, while 44 proteins were identified as differentially expressed spots through MALDI-TOF-MS. Of the identified differential protein spots, 31 were significant according to the bioinformatics analysis results. Proteasome subunit alpha type-1 (PSMA1) expression was down-regulated in HCC TIF. Thus, PSMA1 is considered as a potential biomarker for HBV-associated hepatocellular carcinoma.

Keywords: Hepatocellular carcinoma; proteasome subunit alpha type-1; tissue interstitial fluid.