Hydrogen Peroxide-Generating Electrochemical Scaffold Activity against Trispecies Biofilms

Antimicrob Agents Chemother. 2020 Mar 24;64(4):e02332-19. doi: 10.1128/AAC.02332-19. Print 2020 Mar 24.

Abstract

The antibiofilm activity of a hydrogen peroxide-generating electrochemical scaffold (e-scaffold) was determined against mono- and trispecies biofilms of methicillin-resistant Staphylococcus aureus, multidrug-resistant Pseudomonas aeruginosa, and Candida albicans Significant time-dependent decreases were found in the overall CFU of biofilms of all three monospecies and the trispecies forms. Confocal laser scanning microscopy showed dramatic reductions in fluorescence intensities of biofilm matrix protein and polysaccharide components of e-scaffold-treated biofilms. The described e-scaffold has potential as a novel antibiotic-free strategy for treating wound biofilms.

Keywords: Candida albicans; Pseudomonas aeruginosa; Staphylococcus aureus; biofilm; e-scaffold; hydrogen peroxide.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Anti-Infective Agents / chemistry
  • Anti-Infective Agents / pharmacology*
  • Biofilms* / drug effects
  • Candida albicans / drug effects
  • Colony Count, Microbial
  • Drug Delivery Systems / methods
  • Electrochemical Techniques / instrumentation
  • Electrochemical Techniques / methods*
  • Extracellular Matrix Proteins / metabolism
  • Hydrogen Peroxide / chemistry
  • Hydrogen Peroxide / metabolism*
  • Methicillin-Resistant Staphylococcus aureus / drug effects
  • Microscopy, Confocal
  • Polysaccharides / metabolism
  • Pseudomonas aeruginosa / drug effects
  • Time Factors

Substances

  • Anti-Infective Agents
  • Extracellular Matrix Proteins
  • Polysaccharides
  • Hydrogen Peroxide