Effect of Cultivar on Chlorophyll Meter and Canopy Reflectance Measurements in Cucumber

Sensors (Basel). 2020 Jan 16;20(2):509. doi: 10.3390/s20020509.

Abstract

Optical sensors can be used to assess crop N status to assist with N fertilizer management. Differences between cultivars may affect optical sensor measurement. Cultivar effects on measurements made with the SPAD-502 (Soil Plant Analysis Development) meter and the MC-100 (Chlorophyll Concentration Meter), and of several vegetation indices measured with the Crop Circle ACS470 canopy reflectance sensor, were assessed. A cucumber (Cucumis sativus L.) crop was grown in a greenhouse, with three cultivars. Each cultivar received three N treatments, of increasing N concentration, being deficient (N1), sufficient (N2) and excessive (N3). There were significant differences between cultivars in the measurements made with both chlorophyll meters, particularly when N supply was sufficient and excessive (N2 and N3 treatments, respectively). There were no consistent differences between cultivars in vegetation indices. Optical sensor measurements were strongly linearly related to leaf N content in each of the three cultivars. The lack of a consistent effect of cultivar on the relationship with leaf N content suggests that a unique equation to estimate leaf N content from vegetation indices can be applied to all three cultivars. Results of chlorophyll meter measurements suggest that care should be taken when using sufficiency values, determined for a particular cultivar.

Keywords: genotype; greenhouse; leaf nitrogen; proximal optical sensors; vegetation index.

MeSH terms

  • Chlorophyll / analysis*
  • Crops, Agricultural / chemistry*
  • Cucumis sativus / chemistry*
  • Fertilizers
  • Nitrogen / analysis
  • Optical Imaging / methods*
  • Phenotype
  • Plant Leaves / chemistry

Substances

  • Fertilizers
  • Chlorophyll
  • Nitrogen