A Mycoplasma Genomic DNA Probe using Gated Nanoporous Anodic Alumina

Luís Pla; Elisabet Xifré-Pérez; Àngela Ribes; Elena Aznar; M Dolores Marcos; Lluís F Marsal; Ramón Martínez-Máñez; Félix Sancenón

doi:10.1002/cplu.201600651

A Mycoplasma Genomic DNA Probe using Gated Nanoporous Anodic Alumina

Chempluschem. 2017 Mar;82(3):337-341. doi: 10.1002/cplu.201600651. Epub 2017 Jan 12.

Authors

Luís Pla^{1

2

3}, Elisabet Xifré-Pérez⁴, Àngela Ribes^{1

2

3}, Elena Aznar^{1

3}, M Dolores Marcos^{1

2

3}, Lluís F Marsal⁴, Ramón Martínez-Máñez^{1

2

3}, Félix Sancenón^{1

2

3}

Affiliations

¹ Departamento de Química, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022, Valencia, Spain.
² Instituto Interuniversitario de Investigación de Reconocimiento Molecular, y Desarrollo Tecnológico (IDM), Universitat Politécnica de Valencia, Universitat de Valencia, Camino de Vera, s/n, 46022, Valencia, Spain.
³ CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN).
⁴ Departamento de Ingeniería Electrónica, Eléctrica y Automática, Universidad Rovira i Virgili, Avda. Paissos Catalans 26, 43007, Tarragona, Spain.

PMID: 31962016
DOI: 10.1002/cplu.201600651

Abstract

A nanoporous anodic alumina (NAA)-based sensor system for the detection of Mycoplasma was developed through the implementation of "molecular gates" selective to the presence of this bacterium. The capped support showed a negligible cargo release, while presence of Mycoplasma genomic DNA resulted in the release of rhodamine B fluorescent dye. This sensor system presents a limit of detection of 20 genomic DNA copies⋅μL^-1 and was applied to the detection of Mycoplasma bacteria in competitive environments, such as culture cell media.

Keywords: DNA; Mycoplasma detection; gated materials; nanoporous anodic alumina.

Abstract

Grants and funding