Label free-based proteomic analysis of Escherichia coli O157:H7 subjected to ohmic heating

Abstract

To investigate the inactivation mechanism of ohmic heating (OH) on Escherichia coli O157:H7 at the same inactivation levels, a label-free quantitative proteomic approach was employed in this study. Quantification of 2633 proteins was obtained with high confidence. Compared to untreated samples (CT), a total of 169, 84, and 26 proteins showed significantly differential abundance after high voltage OH (HVOH, 10 V/cm), low voltage OH (LVOH, 5 V/cm), and water bath heating (WB), respectively. Glyoxylate and dicarboxylate metabolism, ABC transporters, biosynthesis of amino acids, glycerophospholipid metabolism, and ribosome pathway were the main KEGG pathways mediated by OH, but only ribosome pathway was greatly affected by WB. The significant differences in proteome changes of E. coli O157:H7 among HVOH, LVOH, and WB treatments, especially the greater number of differential proteins in HVOH, indicated that OH might exert additional effects on proteome of E. coli O157:H7 due to the electric current, particularly in HVOH with higher electric field. This result enriched our understanding of molecular changes of E. coli O157:H7 induced by OH and provided data reference for further research into the inactivation mechanism of OH.

Keywords: 50S ribosomal protein L36 (15833418); Aldehyde dehydrogenase (GI: 15831275); DNA-J (GI: 15829314); DNA-binding protein (GI: 15833852); Escherichia coli O157:H7; HEAT shock chaperone IbpB (GI: 162139744); HPLC-MS/MS; Inactivation mechanism; Ohmic heating; Oligopeptide transporter permease (GI: 15830998); Outer membrane porin protein C (GI: 15832358); RNA polymerase sigma factor RpoE (GI: 15832693); Response regulator of RpoS (GI: 15830991); Water bath heating; Zinc transporter (GI: 15831180).