Introduction: This study aims to determine the genital HPV prevalence in reproductive-age women in Thua Thien Hue Province and comparison with HPV incidence in Hue University Hospital, Vietnam.
Methodology: Cross-sectional study on 1,034 women of reproductive age from 11 communes/wards of three districts representing three different geographic areas of Thua Thien Hue Province, Vietnam. The hospital-based group included 102 women with cervicitis and/or abnormal Pap smear result coming to Hue University Hospital. Extracting DNA from cervical samples, performing the real-time PCR for detecting HPV and the reverse dot-blot assay for HPV typing in HPV positive cases.
Results: In community, HPV prevalence was 0.9%. Mean-age of HPV positive group was 37.9 ± 6.2 years. The detected low-risk types were 6 and 11; high-risk types include 16, 18, 33, 45, 52, and 58. Single-type infection was found in 66.7% of cases. In hospital-based group, 41.2% of women have been infected with HPV, 6 different HPV types were detected. HPV18 was the most frequent high-risk type (33.3%), followed by HPV16 (15.1%); HPV6 was the most frequent among low-risk HPV types (31.2%). Single-type infection rate was 33,3%; 2 and 3 types co-infections were 28,6% and 38.1%, respectively.
Conclusions: Routine screening of high-risk HPV infection in women with symptomatic gynecologic infection and/or abnormal Pap smear appears to be benefit in early detection and prevention of cervical cancer, due to the high incidence of HPV infection.
Keywords: HPV; RT-PCR; Reverse dot-blot; genital tract infection; real-time PCR.
Copyright (c) 2018 Vu Quoc Huy Nguyen, Viet Quynh Tram Ngo, Minh Tam Le, Hoang Bach Nguyen, Quang Vinh Truong, Ngoc Thanh Cao, Pietro Cappuccinelli, Van Bao Thang Phan.