Isolation, structure, and potential biotechnological applications of the exopolysaccharide from Paenibacillus polymyxa 92

Vyacheslav S Grinev; Kristina V Tregubova; Alexander A Anis'kov; Elena N Sigida; Alexander A Shirokov; Yulia P Fedonenko; Irina V Yegorenkova

doi:10.1016/j.carbpol.2019.115780

Isolation, structure, and potential biotechnological applications of the exopolysaccharide from Paenibacillus polymyxa 92

Carbohydr Polym. 2020 Mar 15:232:115780. doi: 10.1016/j.carbpol.2019.115780. Epub 2019 Dec 24.

Authors

Vyacheslav S Grinev¹, Kristina V Tregubova², Alexander A Anis'kov³, Elena N Sigida², Alexander A Shirokov⁴, Yulia P Fedonenko⁴, Irina V Yegorenkova²

Affiliations

¹ Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov 410049, Russia; Chernyshevsky Saratov State University, 83 Ulitsa Astrakhanskaya, Saratov 410012, Russia. Electronic address: grinev@ibppm.ru.
² Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov 410049, Russia.
³ VIC Animal Health, VIC GROUP, 46G Berezovaya Ulitsa, Severny 1, Belgorodskaya Oblast, 8570, Russia.
⁴ Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov 410049, Russia; Chernyshevsky Saratov State University, 83 Ulitsa Astrakhanskaya, Saratov 410012, Russia.

PMID: 31952589
DOI: 10.1016/j.carbpol.2019.115780

Abstract

Paenibacillus polymyxa 92, isolated from wheat roots, produced large amounts (38.4 g L^-1) of exopolysaccharide (EPS) in a liquid nutrient medium containing 10 % (w/v) sucrose. The EPS was precipitated from the culture broth with cold acetone and was purified by gel filtration and anion-exchange chromatography. The molecular mass of the EPS was 2.29-1.10 × 10⁵ Da. Diffuse reflectance infrared Fourier transform and nuclear magnetic resonance spectra showed that the EPS was a linear β-(2→6)-linked fructan (levan). Aqueous EPS solutions showed pseudoplastic behavior when shear stress was applied at different temperatures. By using the Ostwald-de Waele model, the rheological characteristics of the EPS solution were ascertained. The sorption capacity of the EPS for Zn(II), Cd(II), Pb(II), and Cu(II) was investigated. Sorption was maximal (q = 481 mg g^-1) for Cu(II) ions. In model experiments, treatment of wheat seeds with EPS solution significantly increased the length of seedling roots and shoots.

Keywords: Exopolysaccharide; Levan; Paenibacillus polymyxa; Plant-growth-promoting activity; Rheology.

MeSH terms

Adsorption
Biotechnology*
Carbohydrate Conformation
Metals, Heavy / chemistry
Paenibacillus polymyxa / chemistry*
Polysaccharides, Bacterial / chemistry
Polysaccharides, Bacterial / isolation & purification*
Rheology
Triticum / microbiology

Substances

Metals, Heavy
Polysaccharides, Bacterial