Both activated sludge/contact aeration (AS/CA) and AS-only systems for treating oil-containing domestic sewage were tested. The results of these tests indicate that the oil and grease removal ratios of the AS/CA system exceeded those of the AS-only system. When the influent oil and grease concentration reached 60 mg/L, the effluent concentration of the AS system was 13 mg/L, which exceed 10 mg/L, the Taiwan Effluent Standard for oil and grease. However, in the AS/CA system, the effluent oil and grease concentration was 8 mg/L, which was below the required standard. This study analyzes the biological phases of the AS-only system and the combined AS/CA system using a scanning electronic microscope and a denatured gradient gel electrophoresis method when the inflow concentration of oil and grease is increased to 120 mg/L. The results of the Chemical Oxygen Demand (COD) experiment reveal that the AS/CA system is affected less than the AS system, and the COD removal rate of the AS/CA system is maintained at 81%, which exceeds that (61.5%) of the AS-only system. The analytical results thus obtained suggest that both the amounts of biological phase and the biomass in the combined AS/CA system exceed those of an AS-only system.
Keywords: DGGE; SEM; activated sludge/contact aeration; biological phase; oil and grease; true color.