Background: TRIM proteins are important members of E3 ubiquitin ligases, and many studies have confirmed that TRIM family members play an important role in the development of various tumors. We found that TRIM59 expression level in non-small cell lung cancer (NSCLC) was significantly increased through second-generation sequencing. The purpose of this study was to investigate the expression of TRIM59 in NSCLC and its relationship with the clinicopathological parameters as well as the prognosis of patients.
Methods: The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets were excavated to analyze the expression of TRIM59 mRNA in NSCLC and its relationship with the prognosis of patients; The expression of TRIM59 protein in 90 tumor tissues and adjacent tissues was detected by immunohistochemical staining, and the relationship between the expression of TRIM59 protein and clinicopathological parameters and prognosis was analyzed.
Results: Overexpression of TRIM59 mRNA in tumor tissues predicted poor prognosis. The expression level of TRIM59 protein was significantly higher in tumor tissues than in adjacent tissues, and TRIM59 protein expression was correlated with tumor size (P=0.007), tumor differentiation (P=0.009), tumor-node-metastasis (TNM) stage (P=0.003) and lymph node metastasis (P=0.003). Multivariate Cox regression analyses showed that along with TNM stage, overexpression of TRIM59 could be considered an independent prognostic factor for NSCLC patients.
Conclusions: The expression of TRIM59 is closely related to the prognosis of NSCLC patients, and it is an independent risk factor for NSCLC patients.
【中文题目：TRIM59在非小细胞肺癌中的表达及其与预后的关系】 【中文摘要：背景与目的 TRIM家族蛋白是E3泛素连接酶的重要成员，诸多研究证实TRIM家族成员在多种肿瘤的发生发展中发挥了重要作用。前期我们通过高通量测序发现TRIM59 mRNA在非小细胞肺癌组织中表达明显升高。本研究旨在探讨TRIM59在非小细胞肺癌中的表达变化及其与患者临床病理特征和预后的关系。方法 检索高通量芯片表达谱数据库（Gene Expression Omnibus, GEO）和肿瘤基因组计划（The Cancer Genome Atlas, TCGA）数据库中的非小细胞肺癌基因表达谱数据和临床数据，分析TRIM59 mRNA的表达与非小细胞肺癌患者预后的关系；采用免疫组织化学染色的方法检测TRIM59蛋白在90例肺癌组织及癌旁组织中表达，分析TRIM59蛋白的表达与患者临床病理参数及预后的关系。结果 TRIM59 mRNA在肺癌组织中表达明显升高并提示预后不良；TRIM59蛋白在肺癌组织中高表达，在癌旁组织低表达或不表达，TRIM59蛋白的表达与肿瘤大小（P=0.007）、病理分级（P=0.009）、肿瘤原发灶-淋巴结-转移（tumor-node-metastasis, TNM）分期（P=0.003）和淋巴结转移（P=0.003）密切相关。TRIM59蛋白高表达的患者预后较差，Cox多因素回归分析发现TRIM59蛋白的表达和TNM分期是影响患者预后的独立危险因素。结论 TRIM59的表达水平与非小细胞肺癌的预后密切相关，是影响患者预后的独立危险因素。】 【中文关键词：TRIM59；肺肿瘤；预后】.
Keywords: Lung neoplasms; Prognosis; TRIM59.