Downregulation of HNRNPK in human cancer cells inhibits lung metastasis

Mengyuan Li; Wenlong Zhang; Xingjiu Yang; Hongfei Liu; Lin Cao; Weisha Li; Le Wang; Guoxin Zhang; Ran Gao

doi:10.1002/ame2.12090

Downregulation of HNRNPK in human cancer cells inhibits lung metastasis

Animal Model Exp Med. 2019 Nov 21;2(4):291-296. doi: 10.1002/ame2.12090. eCollection 2019 Dec.

Authors

Mengyuan Li^{1

2}, Wenlong Zhang^{1

2}, Xingjiu Yang^{1

2}, Hongfei Liu^{1

2}, Lin Cao^{1

2}, Weisha Li^{1

2}, Le Wang^{1

2}, Guoxin Zhang^{1

2}, Ran Gao^{1

2}

Affiliations

¹ Key Laboratory of Human Disease Comparative Medicine (National Health and Family Planning Commission) The Institute of Laboratory Animal Science Chinese Academy of Medical Sciences & Peking Union Medical College Beijing P.R. China.
² Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases Beijing P.R. China.

Abstract

Background: Lung cancer frequently occurs in the clinic, leading to poor prognosis and high mortality. Markers for early diagnosis of lung cancer are scarce, and further potential therapeutic targets are also urgently needed.

Method: We established a new mouse model in which the specific gene HNRNPK (heterogeneous nuclear ribonucleoprotein K) was downregulated after administration of doxycycline. The lung metastatic nodules were investigated using bioluminescence imaging, micro-CT, and autopsy quantification.

Results: Compared with the short hairpin negative control group, less lung metastatic nodules were formed in the short hairpin RNA group.

Conclusion: Downregulation of HNRNPK in cancer cells can inhibit lung metastasis.

Keywords: HNRNPK; lung metastasis; mouse model.