MiR-659-3p regulates the progression of chronic myeloid leukemia by targeting SPHK1

Zhigang Liu; Chuan He; Ying Qu; Xinchuan Chen; Huanling Zhu; Bing Xiang

MiR-659-3p regulates the progression of chronic myeloid leukemia by targeting SPHK1

Int J Clin Exp Pathol. 2018 May 1;11(5):2470-2478. eCollection 2018.

Authors

Zhigang Liu¹, Chuan He¹, Ying Qu¹, Xinchuan Chen¹, Huanling Zhu¹, Bing Xiang¹

Affiliation

¹ Department of Hematology, Hematology Research Laboratory, West China Hospital, Sichuan University Chengdu 610041, China.

PMID: 31938359
PMCID: PMC6958280

Abstract

Accumulating evidence shows that microRNAs (miRNAs) are significant regulators of multiple cellular processes including chronic myelocytic leukemia (CML). However, the mechanism of miR-659-3p in CML remains unclear. In this study, we aimed to investigate the potential role of miR-659-3p in CML progression. We found that miR-659-3p was down-regulated in CML. The upregulation of miR-659-3p significantly suppressed the proliferation ability, and enhanced the apoptosis capacity of K562 cells. Simultaneously, we found that sphingosine kinase 1 (SPHK1) was up-regulated in CML. MiR-659-3p performed its function through downregulating SPHK1 by binding to its untranslated region (3'-UTR). These results suggested that miR-659-3p, acted as a tumor suppressor, decreased the proliferation ability of K562 cells, and increased the apoptosis capacity of K562 cells. Therefore, our study provided a new theoretical basis of miR-659-3p which may be a promising approach for CML treatment.

Keywords: SPHK1; chronic myeloid leukemia; miR-659-3p.