Roots and Leaf Extracts of Dipsacus fullonum L. and Their Biological Activities

Jan Oszmiański; Aneta Wojdyło; Piotr Juszczyk; Paulina Nowicka

doi:10.3390/plants9010078

Roots and Leaf Extracts of Dipsacus fullonum L. and Their Biological Activities

Plants (Basel). 2020 Jan 8;9(1):78. doi: 10.3390/plants9010078.

Authors

Jan Oszmiański¹, Aneta Wojdyło¹, Piotr Juszczyk², Paulina Nowicka¹

Affiliations

¹ Department of Fruit, Vegetable and Plant Nutraceuticals Technology, Wrocław University of Environmental and Life Sciences, 37 Chełmońskiego Street, 51-630 Wrocław, Poland.
² Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, 37 Chełmońskiego Street, 51-630 Wrocław, Poland.

Abstract

The aim of the study was to identify and evaluate the content of iridoids and phenolic compounds in the leaves and roots of Dipsacus fullonum L. They were identified and quantified by UPLC-PDA-MS/MS. Five iridoid compounds (loganic acid, loganin, sweroside, cantleyoside, and sylvestroside III) were identified in Dipsacus fullonum L. leaves and roots. Seven phenolic acids and three flavones were identified in the leaves, and seven phenolic acids were detected in the roots. The leaves contained more iridoids and phenolic compounds than the roots. We also evaluated the antimicrobial (anti-bacterial and anti-yeast), antioxidant (ORAC methods), and antiacetylcholinesterase (AChE) activities of Dipsacus fullonum L. leaves and roots. Leaf extract demonstrated the strongest antioxidant activity, but roots showed stronger antiacetylcholinesterase activity than leaves. The study also confirmed antibacterial activity of root-derived compounds against Staphylococcus aureus DSM 799 and Escherichia coli ATCC 10536.

Keywords: Dipsacus fullonum L.; UPLC-PDA-QTof-MS/MS; anti-acetylcholinesterase activities; anti-bacterial; anti-oxidant; anti-yeast; irydoids; polyphenols.