CD147 is a transmembrane glycoprotein that when highly expressed contributes to tumor progression. In the present study, we investigate the clinical relevance of CD147 expression in CCSC tissues and evaluate the association between CD147 expression and cervical lymph node metastasis; CD147 was detected using immunohistochemistry. To functionally analyze the role of CD147 in CCSC cell lines in vitro, SiHa cells were employed, whose endogenous CD147 was artificially downregulated, by using lentiviral-based transfection. Moreover, we have confirmed that knockdown of CD147 led to reduced levels of cellular lipid content in shCD147 cells by BODIPY staining. Cell invasion and migration were analyzed using transwell assays and wound healing. Angiogenesis and lymphangiogenesis were assessed by an endothelial cell tube formation assay. Our data showed that highly expressed CD147 up-regulated the major lipogenic genes, FAS and ACC1 to promote de novo lipogenesis, and knockdown of CD147 significantly inhibited the migration and invasion of CSCC cells. The culture supernatants of CD147 knockdown cells significantly inhibited vascular and lymphatic endothelial cell tube formation. Our results suggest that CD147-mediated FAS and ACC1 overexpression are major regulators of cervical cancer growth and metastasis.
Keywords: CD147; Cervical cancer; fatty acid synthase; invasion; migration.
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