Effect of ceftiofur on mesophilic anaerobic digestion of dairy manure and the reduction of the cephalosporin-resistance gene cmy-2

Daniel Flores-Orozco; Rakesh Patidar; David B Levin; Richard Sparling; Ayush Kumar; Nazim Çiçek

doi:10.1016/j.biortech.2019.122729

Effect of ceftiofur on mesophilic anaerobic digestion of dairy manure and the reduction of the cephalosporin-resistance gene cmy-2

Bioresour Technol. 2020 Apr:301:122729. doi: 10.1016/j.biortech.2019.122729. Epub 2020 Jan 2.

Authors

Daniel Flores-Orozco¹, Rakesh Patidar², David B Levin³, Richard Sparling⁴, Ayush Kumar⁵, Nazim Çiçek⁶

Affiliations

¹ Department of Biosystems Engineering, University of Manitoba, Winnipeg, Manitoba R3T 5V6, Canada. Electronic address: floresod@myumanitoba.ca.
² Department of Microbiology, University of Manitoba, Winnipeg, Manitoba R3T 5V6, Canada. Electronic address: Rakesh.Patidar@umanitoba.ca.
³ Department of Biosystems Engineering, University of Manitoba, Winnipeg, Manitoba R3T 5V6, Canada. Electronic address: David.Levin@umanitoba.ca.
⁴ Department of Microbiology, University of Manitoba, Winnipeg, Manitoba R3T 5V6, Canada. Electronic address: Richard.Sparling@umanitoba.ca.
⁵ Department of Microbiology, University of Manitoba, Winnipeg, Manitoba R3T 5V6, Canada. Electronic address: Ayush.Kumar@umanitoba.ca.
⁶ Department of Biosystems Engineering, University of Manitoba, Winnipeg, Manitoba R3T 5V6, Canada. Electronic address: Nazim.Cicek@umanitoba.ca.

PMID: 31931335
DOI: 10.1016/j.biortech.2019.122729

Abstract

The effect of ceftiofur (CEF), a commonly used antibiotics on dairy farms, on the performance and stability of mesophilic batch anaerobic digestion (AD) of dairy manure was evaluated in terms of methane production, organic matter removal (COD, dCOD, TS, and VS), and synthesis of end-products (VFAs, CO₂, and H₂). The results indicated that only CEF concentrations of 10 mg/L or higher significantly affected the performance of the AD process, although the overall stability was not compromised. Biochemical analyses suggested that hydrolytic microorganisms were the most affected by the presence of CEF leading to lower COD removal, whereas acetogens were only temporarily slowed down. Methanogens, on the other hand, were not directly affected by any of the CEF concentrations tested (0.2-250 mg/L). Additionally, the presence of CEF was shown to alter the incidence of the cephalosporin-resistance marker, cmy-2, although an overall reduction was achieved in 15-day batch anaerobic digestion trials.

Keywords: Anaerobic digestion; Antibiotic-resistance; Biogas; Ceftiofur; Dairy manure.

MeSH terms

Anaerobiosis
Biofuels*
Bioreactors
Cephalosporins
Manure*
Methane

Substances

Biofuels
Cephalosporins
Manure
ceftiofur
Methane