Jasmonic acid (JA) is an important phytohormone, which among others may be involved in the regulation of plant accumulating heavy metal. This experiment was designed to explore the effects of exogenous JA on the responses of alfalfa to Cu stress (100 μM) in Hoagland solution. When 1, 5 or 10 mM JA was added to the treatment with Cu addition, Cu concentrations in roots and leaves of alfalfa were significantly decreased (p < 0.05) to some extents compared to the treatment without JA addition. The biomasses of roots and leaves of alfalfa in treatments of JA additions were significantly increased (p < 0.05) compared to the Cu stress treatment. Similarly, the concentrations of Chlorophyll, antioxidant enzyme activities, MDA and H2O2 were improved accordingly. But these factors of JA were not improved further when its concentration added in media was the highest (10 mM), indicating its improvement roles were limited. These results suggested that there were positive roles of exogenous JA on alfalfa decreased its Cu accumulation and toxicities might via reduced oxidative stress.
Keywords: Biomass; Chlorophyll; Forage grass; Heavy metal; Plant phytohormone.
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