Anti-complementary activity of a degraded sulfated heterogalactan from red alga Pyropia haitanensis

Zhongshan Zhang; Xiaomei Wang; Fang Lv; Xiaochao Xie; Shaoyong Zhang; Chuner Cai; Rui Jia; Yongliang Pan; Feng Liu

doi:10.1016/j.ijbiomac.2020.01.045

Anti-complementary activity of a degraded sulfated heterogalactan from red alga Pyropia haitanensis

Int J Biol Macromol. 2020 Mar 15:147:527-533. doi: 10.1016/j.ijbiomac.2020.01.045. Epub 2020 Jan 10.

Authors

Zhongshan Zhang¹, Xiaomei Wang², Fang Lv³, Xiaochao Xie⁴, Shaoyong Zhang², Chuner Cai⁵, Rui Jia⁵, Yongliang Pan², Feng Liu⁶

Affiliations

¹ Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University, Huzhou 313000, China. Electronic address: 01959@zjhu.edu.cn.
² Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University, Huzhou 313000, China.
³ Marine Biology Institute of Shandong Province, Qingdao 266104, China.
⁴ Hiser Medical Center of Qingdao, Qingdao, Shandong 266033, China.
⁵ College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai 201306, China.
⁶ CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong 266071, China.

PMID: 31926924
DOI: 10.1016/j.ijbiomac.2020.01.045

Abstract

A sulfated galactan LP-G2 was isolated and purified from red alga, Pyropia haitanensis. It was a branched polysaccharide with average molecular weight about 8381 Da, composed of Gal, Glc, GalA and Ara. The structure of LP-G2 was determined using IR and NMR spectroscopy. It was composed of →4)-β-d-galactose→4)-α-l-galactose-6- sulfate segments, with β-d-Glc and α-d-galactose unit substituted at the 6-position of α-l-galactose. Functional analysis showed that LP-G2 inhibited complement activation on both the classic and alternative pathways with CH₅₀ value of 3.08 ± 0.25 mg/mL and AP₅₀ value of 2.23 ± 0.20 mg/mL, respectively. Preliminary mechanism studies by using complement component depleted-sera indicated that LP-G2 selectively interacts with C1q, C2, C4 and C9. The results suggested that LP-G2 could be of potential benefits in treatment of the complement associated diseases.

Keywords: Anti-complementary; Pyropia haitanensis; Sulfated heterogalactan.

MeSH terms

Animals
Complement Activation / drug effects*
Complement System Proteins
Galactans* / chemistry
Galactans* / isolation & purification
Galactans* / pharmacology
Guinea Pigs
Rhodophyta / chemistry*

Substances

Galactans
Complement System Proteins