Cytotoxicity and inflammatory response of different types of provisional restorative materials

Marcio Campaner; Aline Satie Takamiya; Sandro Basso Bitencourt; Leticia Cerri Mazza; Sandra Helena Penha de Oliveira; Ricardo Shibayama; Valentim Adelino Ricardo Barão; Cortino Sukotjo; Aldiéris Alves Pesqueira

doi:10.1016/j.archoralbio.2019.104643

Cytotoxicity and inflammatory response of different types of provisional restorative materials

Arch Oral Biol. 2020 Mar:111:104643. doi: 10.1016/j.archoralbio.2019.104643. Epub 2019 Dec 26.

Authors

Marcio Campaner¹, Aline Satie Takamiya², Sandro Basso Bitencourt¹, Leticia Cerri Mazza¹, Sandra Helena Penha de Oliveira³, Ricardo Shibayama⁴, Valentim Adelino Ricardo Barão⁵, Cortino Sukotjo⁶, Aldiéris Alves Pesqueira⁷

Affiliations

¹ Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, Brazil.
² Department of Surgery and Integrated Clinic, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, Brazil.
³ Department of Basic Sciences, São Paulo State University (UNESP), School of Dentistry, Araçatuba, Brazil.
⁴ Department of Restorative Dentistry, University State of Londrina (UEL), Londrina, Parana, Brazil.
⁵ Department of Prosthodontics and Periodontology, University of Campinas (UNICAMP), Piracicaba Dental School, Piracicaba, São Paulo, Brazil.
⁶ Department of Restorative Dentistry, University of Illinois at Chicago, College of Dentistry, Chicago, IL, USA.
⁷ Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, Brazil. Electronic address: aldieris.ap@foa.unesp.br.

PMID: 31918345
DOI: 10.1016/j.archoralbio.2019.104643

Abstract

Aim: To evaluate the cytotoxicity and inflammatory response of different types of provisional restorative materials to mice gingival fibroblasts.

Methods: Cytotoxicity of provisional material discs (thermal-polymerized acrylic resin; auto-polymerized acrylic resin; bisacrylic resin; nano-ceramic resin for CAD/CAM and prefabricated polymer block for CAD/CAM) to Mice (Balb/c) gingival cell were investigated under direct and indirect contact (extracts) at 24, 48 and 72 h, using the MTT and Alamar blue assays. Materials extracts (24 h) were applied to the cell culture (indirect contact) or cells were seeded on discs of provisional materials, and the cytotoxicity and production of IL-6, IL-1β and TNF-α after 24, 48 and 72 h were analyzed through MTT, Alamar Blue® and ELISA. Culture medium was used as control for indirect contact assay (extract) and the surfaces of the wells without discs of provisional materials were used as control for direct contact assay. Results were analysed statistically by ANOVA followed by the Bonferroni-Test correction. Statistically significant differences were considered if P was < .05.

Results: Auto-polymerized and bisacrylic resins (direct contact) reduced cell viability after 24, 48 and 72 h compared to control (P < .05). Indirect contact (extract) was not cytotoxic to cells at all periods compared to control (P > .05). Auto-polymerized and bisacrylic resins increased IL-6, IL-1β and TNF-α levels mainly at 24 h when compared to the other materials (P < .001).

Conclusion: Auto-polymerized and bisacrylic were more cytotoxic to mice gingival fibroblasts. CAD/CAM nano ceramic resin and prefabricated polymer blocks are more predictable materials to preserve the periodontal soft tissues.

Keywords: Acrylic resins; Computer-aided design; Fixed partial denture; Materials testing.

MeSH terms

Acrylic Resins*
Animals
Ceramics
Composite Resins
Computer-Aided Design
Dental Materials*
Materials Testing
Mice
Surface Properties

Substances

Acrylic Resins
Composite Resins
Dental Materials