Effects of DBD air plasma treatment on the enhancement of black gram (Vigna mungo l.) seed germination and growth

M Billah; S A Sajib; N C Roy; M M Rashid; M A Reza; M M Hasan; M R Talukder

doi:10.1016/j.abb.2020.108253

Effects of DBD air plasma treatment on the enhancement of black gram (Vigna mungo l.) seed germination and growth

Arch Biochem Biophys. 2020 Mar 15:681:108253. doi: 10.1016/j.abb.2020.108253. Epub 2020 Jan 7.

Authors

M Billah¹, S A Sajib¹, N C Roy², M M Rashid², M A Reza¹, M M Hasan³, M R Talukder⁴

Affiliations

¹ Molecular Biology and Protein Science Lab, Department of Genetic Engineering & Biotechnology, University of Rajshahi, Rajshahi, 6205, Bangladesh.
² Plasma Science and Technology Lab, Department of Electrical & Electronic Engineering, University of Rajshahi, Rajshahi, 6205, Bangladesh.
³ Plant Pathology Lab, Department of Agronomy & Agricultural Extension, University of Rajshahi, Rajshahi, 6205, Bangladesh.
⁴ Plasma Science and Technology Lab, Department of Electrical & Electronic Engineering, University of Rajshahi, Rajshahi, 6205, Bangladesh. Electronic address: mrtalukder@ru.ac.bd.

PMID: 31917117
DOI: 10.1016/j.abb.2020.108253

Abstract

Effects of black gram (vigna mungo L.cv. Barimash 3) seed treatments with 400 torr dielectric barrier discharge (DBD) air plasma on seed surface morphology, seed germination, seedling growth and antioxidant enzyme activities in the roots, shoots and leaves were investigated. The plasma discharge voltage, frequency, electrode spacing, gas temperature and power were 5kV, 4.5kHz, 60mm, 310K and 45W, respectively. The seeds were treated for the duration ranging from 20 to 180 s. Seed germination rate, seedling growth, total chlorophyll content, total soluble protein and sugar concentrations in the seedlings grown from the treated seeds were found to increase 13.67%, 37.13%, 37.26%,53.60% and 51.71%, respectively, with respect to control. This study reveals that the DBD air plasma was involved in the enhancement of nitrogen complex in the seed coat of black gram which upregulated the protein through nitrogen conversion that was ultimately responsible for the increased seed germination and seedling growth of black gram.

Keywords: DBD plasma; Enzymatic activity; H(2)O(2); NO; Seed germination; Seedling growth; Vigna mungo (black gram).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Equipment Design
Germination* / drug effects
Hydrogen Peroxide / metabolism
Nitric Oxide / metabolism
Plasma Gases / metabolism*
Seedlings / drug effects
Seedlings / growth & development
Seeds / drug effects
Seeds / growth & development*
Vigna / drug effects
Vigna / growth & development*

Substances

Plasma Gases
Nitric Oxide
Hydrogen Peroxide