Physicochemical and Antioxidant Properties of the Degradations of Polysaccharides from Dendrobium officinale and Their Suitable Molecular Weight Range on Inducing HeLa Cell Apoptosis

Xiaofeng Zhang; Yingyi Luo; Gang Wei; Yunrong Li; Yuechun Huang; Jiahui Huang; Chenxing Liu; Runping Huang; Guoxiong Liu; Zhaofeng Wei; Shuxiu Du

doi:10.1155/2019/4127360

Physicochemical and Antioxidant Properties of the Degradations of Polysaccharides from Dendrobium officinale and Their Suitable Molecular Weight Range on Inducing HeLa Cell Apoptosis

Evid Based Complement Alternat Med. 2019 Aug 28:2019:4127360. doi: 10.1155/2019/4127360. eCollection 2019.

Authors

Xiaofeng Zhang¹, Yingyi Luo¹, Gang Wei^{1

2}, Yunrong Li³, Yuechun Huang⁴, Jiahui Huang¹, Chenxing Liu¹, Runping Huang¹, Guoxiong Liu^{2

5}, Zhaofeng Wei^{2

6}, Shuxiu Du^{2

7}

Affiliations

¹ School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
² Shaoguan Institute of Danxia Dendrobium Officinale, Shaoguan 512005, China.
³ Guangxi International Zhuang Medicine Hospital, The Affiliated International Zhuang Medicine Hospital, Guangxi Traditional Chinese Medical University, Nanning 530201, China.
⁴ The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China.
⁵ Shaoguan Hejiantang Ecological Agriculture Company Ltd., Shaoguan 512000, China.
⁶ Shaoguan Danxia Mountain Engineering Center of Dendrobium Technology, Shaoguan 512000, China.
⁷ Shaoguan Runhu Ecological Agriculture Company Ltd., Shaoguan 512000, China.

Abstract

Different molecular weight polysaccharides of Dendrobium officinale (DOPs) have gradually attracted attention because of their broad biological activities. They, however, remain poorly defined whether their antitumor activity is associated with molecular weight. In this study, the physicochemical, antioxidant, and antitumor properties of DOPs, including the crude polysaccharide (DOP) and its six degradation fractions (DOP1-DOP6) extracted from Dendrobium officinale, were determined. Consequently, DOPs were mainly composed of different ratios of mannose and glucose as follows: 5.15 : 1, 4.62 : 1, 4.19 : 1, 4.46 : 1, 4.32 : 1, 4.29 : 1, and 4.23 : 1, and their molecular weights were significantly different ranging from 652.29 kDa to 11.10 kDa. With the concentration increase of DOPs, the scavenging capacity against OH and DPPH free radicals increased. The antitumor ability of DOPs was different that DOP1-DOP5 (Mw: 176.29 kDa-28.48 kDa) exhibited the best antiproliferation activity than DOP (Mw: 652.29 kDa) and DOP6 (Mw: 11.10 kDa) in HeLa cells rather than PC9, A549, and HepG2 cells. Moreover, it is worth mentioning that DOP1 and DOP5 showed stronger capability on inducing apoptosis of HeLa cells than DOP and DOP6 via the mitochondrial pathway by upregulating the ratio of the Bax/Bal-2 mRNA expression. The results demonstrated that DOPs can be used as the potential natural antioxidant and antitumor products in pharmaceutical industries, and the molecular weight is a crucial influential factor of their antitumor activity that 28.48 kDa-176.29 kDa is a suitable range we may refer to.