Interplay of mRNA capping and transcription machineries

Zaur M Kachaev; Lyubov A Lebedeva; Eugene N Kozlov; Yulii V Shidlovskii

doi:10.1042/BSR20192825

Interplay of mRNA capping and transcription machineries

Biosci Rep. 2020 Jan 31;40(1):BSR20192825. doi: 10.1042/BSR20192825.

Authors

Zaur M Kachaev¹, Lyubov A Lebedeva¹, Eugene N Kozlov¹, Yulii V Shidlovskii^{1

2}

Affiliations

¹ Laboratory of Gene Expression Regulation in Development, Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
² Department of Biology and General Genetics, I.M. Sechenov First Moscow State Medical University, Moscow, Russia.

Abstract

Early stages of transcription from eukaryotic promoters include two principal events: the capping of newly synthesized mRNA and the transition of RNA polymerase II from the preinitiation complex to the productive elongation state. The capping checkpoint model implies that these events are tightly coupled, which is necessary for ensuring the proper capping of newly synthesized mRNA. Recent findings also show that the capping machinery has a wider effect on transcription and the entire gene expression process. The molecular basis of these phenomena is discussed.

Keywords: capping checkpoint; mRNA capping; promoter; transcription; transcription pausing.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Gene Expression Regulation
Humans
Models, Genetic*
Promoter Regions, Genetic
RNA Caps / biosynthesis*
RNA Caps / genetics
RNA Polymerase II / metabolism*
RNA, Messenger / biosynthesis*
RNA, Messenger / genetics
Transcription Factors / genetics
Transcription Factors / metabolism
Transcription, Genetic*

Substances

RNA Caps
RNA, Messenger
Transcription Factors
RNA Polymerase II