Measuring local-directional resolution and local anisotropy in cryo-EM maps

Nat Commun. 2020 Jan 2;11(1):55. doi: 10.1038/s41467-019-13742-w.

Abstract

The introduction of local resolution has enormously helped the understanding of cryo-EM maps. Still, for any given pixel it is a global, aggregated value, that makes impossible the individual analysis of the contribution of the different projection directions. We introduce MonoDir, a fully automatic, parameter-free method that, starting only from the final cryo-EM map, decomposes local resolution into the different projection directions, providing a detailed level of analysis of the final map. Many applications of directional local resolution are possible, and we concentrate here on map quality and validation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Anisotropy
  • Cryoelectron Microscopy / methods*
  • Image Processing, Computer-Assisted / methods*
  • Proteasome Endopeptidase Complex / chemistry
  • Ribosomes / chemistry
  • beta-Galactosidase / chemistry

Substances

  • beta-Galactosidase
  • Proteasome Endopeptidase Complex