A dual-modal fluorometric and colorimetric nanoprobe based on graphitic carbon nitrite quantum dots and Fe (II)-bathophenanthroline complex for detection of nitrite in sausage and water

Yuelin Kong; Qiao Cheng; Yu He; Yili Ge; Jiangang Zhou; Gongwu Song

doi:10.1016/j.foodchem.2019.126089

A dual-modal fluorometric and colorimetric nanoprobe based on graphitic carbon nitrite quantum dots and Fe (II)-bathophenanthroline complex for detection of nitrite in sausage and water

Food Chem. 2020 May 15:312:126089. doi: 10.1016/j.foodchem.2019.126089. Epub 2019 Dec 23.

Authors

Yuelin Kong¹, Qiao Cheng¹, Yu He², Yili Ge¹, Jiangang Zhou³, Gongwu Song⁴

Affiliations

¹ Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China.
² Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China; Hubei Province Key Laboratory of Regional Development and Environment Response, Wuhan 430062, China. Electronic address: heyu@hubu.edu.cn.
³ Hubei Province Key Laboratory of Regional Development and Environment Response, Wuhan 430062, China.
⁴ Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China. Electronic address: songgw@hubu.edu.cn.

PMID: 31896452
DOI: 10.1016/j.foodchem.2019.126089

Abstract

A fluorometric and colorimetric dual-mode sensing platform based on graphitic carbon nitrite quantum dots (g-CNQDs) and Fe (II)-bathophenanthroline complex (BPS-Fe²⁺) was designed to the sensitive detection of nitrite (NO₂^-) in sausage and water. In this system, the fluorescence of g-CNQDs was quenched by BPS-Fe²⁺ complex due to the inner filter effect (IFE). When NO₂^- was present, Fe²⁺ was oxidized by nitrite to form BPS-Fe³⁺ complex with BPS, leading to the recovery of the fluorescence from g-CNQDs. Therefore, we constructed a "turn-off-on" fluorescence probe for detection of NO₂^-. Moreover, with the increase of NO₂^- concentration, the color of the solution changed from red to colorless, so the UV-vis measurements and on-site visual detection were realized. The method is capable of detecting NO₂^- in the concentration range of 2.32-34.8 μM with good selectivity and high sensitivity. In addition, the method has the potential to determine NO₂^- in water samples and sausage samples.

Keywords: Colorimetric method; Fluorometric method; Nitrite; Turn-off-on; g-CNQDs.

MeSH terms

Carbon / chemistry*
Colorimetry
Fluorescence
Fluorescent Dyes
Fluorometry
Graphite / chemistry*
Iron Compounds / chemistry
Meat Products / analysis*
Nitrites / chemistry*
Phenanthrolines / chemistry*
Quantum Dots*
Water / chemistry*

Substances

Fluorescent Dyes
Iron Compounds
Nitrites
Phenanthrolines
Water
bathophenanthroline
Carbon
Graphite