Protein-assisted formation of gold clusters-MnO2 nanocomposite for fluorescence imaging of intracellular glutathione

Yanlin Du; Hui Liu; Jiaming Liang; Dongye Zheng; Jiong Li; Shubing Lan; Ming Wu; Aixian Zheng; Xiaolong Liu

doi:10.1016/j.talanta.2019.120524

Protein-assisted formation of gold clusters-MnO₂ nanocomposite for fluorescence imaging of intracellular glutathione

Talanta. 2020 Mar 1:209:120524. doi: 10.1016/j.talanta.2019.120524. Epub 2019 Oct 31.

Authors

Yanlin Du¹, Hui Liu², Jiaming Liang³, Dongye Zheng¹, Jiong Li¹, Shubing Lan¹, Ming Wu⁴, Aixian Zheng⁵, Xiaolong Liu⁶

Affiliations

¹ College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao, Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China; Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, 350002, China.
² The Fifth People's Hospital of Ganzhou, Ganzhou City, 341000, China.
³ School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, China.
⁴ The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao, Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China.
⁵ The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao, Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China. Electronic address: zax040500273@126.com.
⁶ College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao, Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China; Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, 350002, China. Electronic address: xiaoloong.liu@gmail.com.

PMID: 31892049
DOI: 10.1016/j.talanta.2019.120524

Abstract

Herein, a bovine serum albumin-stabilized gold clusters-MnO₂ nanocomposite (BSA@AuNCs-MnO₂) was constructed. Manganese dioxide (MnO₂) was generated in situ on the gold clusters (BSA@AuNCs) based on the redox reaction between bovine serum albumin (BSA) and potassium permanganate (KMnO₄). The fluorescence of BSA@AuNCs can be quenched by the in-situ grown MnO₂, which has strong light absorption ability. It is worth noting that the quenched fluorescence of the BSA@AuNCs can be restored in the presence of glutathione (GSH), and MnO₂ was reduced to Mn²⁺ in return. Encouragingly, 1 μM GSH can cause a detectable fluorescence change. This sensitivity is comparable to other nanomaterials based fluorescent probes. Furthermore, this nanocomposite has obvious superiorities, such as good uniformity, simple preparation and mild reaction. The nanocomposite also has good stability and specificity, which can be further used for visualizing of intracellular GSH.

Keywords: Bovine serum albumin; Fluorescence imaging; Gold clusters; Manganese dioxide; Nanocomposite.